Figure 1From: Detection of dengue group viruses by fluorescence in situ hybridizationSpecific detection of DENV without cross-hybridization towards closely related Flavivirus. Cells were infected with the most closely related Flavivirus YFV and WNV. DENV-1, DENV-2, DENV-3, DENV-4 were used as positive controls. FISH was performed using the three selected probes simultaneously at day 5 post-infection. Positive hybridizing signals (green) are seen in the cytoplasm of C6/36 cells infected with DENV-1 to DENV-4. No hybridizing signals were visible in cells infected with YFV and WNV or in uninfected cells. Host cell nuclei are stained with DAPI. Bar, 10 μm.Back to article page