Pathogen | Gene | Primer sequence (5′-3′) | Size of PCR product (bp) | Reference | Positive controls |
---|---|---|---|---|---|
Borrelia burgdorferi s.l. | OspA | GGG AAT AGG TCT AAT ATT AGC CCAC TAA TTG TTA AAG TGG AAG T | 665 bp | [26] | ATCC BG/V |
Anaplasma phagocytophilum | msp2 | TTG GTC TTG AAG CGC TCG TAATG GAA GGT AGT GTT GGT TAT GGT ATT | 420 bp | [27] | A. phagocytophilum (dog isolate) |
Ehrlichia canis | 16S rRNA | Ehrlichia spp.AGA ACG AAC GCT GGC GGC AAG CCCGT ATT ACC GCG GCT GCT GGC | 478 bp | [29] | E. canis (dog isolate) |
Ehrlichia canis CAA TTA TTT ATA GCC TCT GGC TAT AGCTAT AGG TAC CGT CAT TAT CTT CCC TAT | 389 bp | ||||
Coxiella burnetii | IS1111 | CTC GTA ATC ACC AAT CGC TTC GCAA GAA TGA TCG TAA CGA TGC GC | 337 bp | [28] | 105C. burnetii/ml (culture) |