The synergistic action of imidacloprid and flumethrin and their release kinetics from collars applied for ectoparasite control in dogs and cats

Table 2 The dose response curve showing mean % mortality and efficacy of flumethrin or imidacloprid or a 1: 1.85 combination of flumethrin and imidacloprid coating on glass vial surfaces (mean data from 2 separate trials) against Ctenocephalides felis fleas

	Imidacloprid: Flumethrin 1.85: 1				Flumethrin				Imidacloprid
[μg/cm ² ]	24 h		48 h		24 h		48 h		24 h		48 h
	Mortality	Efficacy	Mortality	Efficacy	Mortality	Efficacy	Mortality	Efficacy	Mortality	Efficacy	Mortality	Efficacy
5	100	100	100	100	75	100	100	100	30	100	100	100
1	100	100	100	100	60	100	80	100	25	90	100	100
0,2	80	100	100	100	40	100	70	100	0	60	80	100
0,04	40	80	60	100	30	60	60	100	0	30	40	100
0,008	0	30	30	60	0	0	0	40	0	0	0	0
0,0016	0	0	0	0	0	0	0	0	0	0	0	0
0,00032	0	0	0	0	0	0	0	0	0	0	0	0
0,000064	0	0	0	0	0	0	0	0	0	0	0	0
min	-1,582	-2,287	-0,078	-0,011	-4,929	-1,243	-3,390	0,031	-1,531	-0,914	-1,752	-0,012
max	100,5	100,4	103,8	100,0	100,0	99,9	93,7	100,0	100,0	101,0	106,0	100,0
ED50 μg/cm²	0,059	0,012	0,033	0,008	0,483	0,010	0,039	0,009	0,537	0,074	0,112	0,017
Slope	1,296	1,285	1,111	3,889	0,363	1,736	1,031	4,006	17,378	1,433	0,937	3,315
ED50 Factor (FI = 1)					8,2	0,9	1,2	1,2	9,1	6,4	3,4	2,3
Normality* Test (Shapiro-Wilk)	Passed	Passed	Passed	Passed	Passed	Passed	Passed	Passed	Passed	Failed	Passed	Passed
P-values* 24 h mortality vs untreated control	0.028				0.004				0.0457
P-values* FI vs F or I	-				0.0094				-0.0024
P-values* F vs I	-				-				-0.0027

* Normality and two-sided paired t-test was perfomed for data used in Figure 4 with all data from both trials, means of the two trials were used for curve fitting and fit statistics using SigmaPlot for Windows 11.0 (Build 11.0.0.75, ^© 2008 Systat Software, Inc.)

ISSN: 1756-3305