Figure 4From: Localization of Wolbachia-like gene transcripts and peptides in adult Onchocerca flexuosa worms indicates tissue specific expressionWestern blot detection of putative peptides with antibodies to peptides corresponding to isotig12596, isotig21532, and Wolbachia HlyD in Onchocerca flexuosa adult worm lysate. Affinity-purified rabbit polyclonal antibodies against a peptide predicted from isotig12596 recognized a band at approximately 50kDa (lane 2). Antibodies raised against a peptide predicted from isotig21532 recognized two main bands at approximately 260 and 130 kDa (lane 4). Antibodies against a peptide from Wolbachia HlyD labeled two main bands at approximately 47 and 18 kDa (lane 6). Purified IgG from the pre-immune serum of the corresponding rabbits did not label these bands (lanes 1, 3 and 5). Approximate molecular masses (kDa) are shown on the left side of the blot.Back to article page