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Table 4 Results of Luminex assay and real time PCR with 15 DNA from stool samples

From: LUMINEX®: a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools

 

E.dispar

E. hist

E. moshk

E hart

E.coli

Real time PCR**

CTL+ Eh_ 1*

−41

981

93

140

−89

-

CTL+ Ed_1

1368

54

−61

214

−79

-

CTL+ Em_ 2

−76

−48

547

−45

−86

-

CTL+ Ehart_1

−82

−87

−80

1844

−85

-

CTL+ Ecoli

−75

−60

−84

−53

1194

-

Sample 1

530

−15

−64

181

−73

E.dispar

Sample 2

772

−10

−74

728

−76

E.dispar

Sample 3

−77

−76

−71

−79

−68

Negative

Sample 4

−44

1094

135

201

59

E. hist

Sample 5

−43

1144

154

185

75

E. hist

Sample 6

597

−4

−75

578

−80

E.dispar

Sample 7

−79

−70

−77

−89

−87

Negative

Sample 8

−50

1027

101

172

44

E. hist

Sample 9

−39

1144

117

197

49

E. hist

Sample10

−39

1145

172

255

97

E. hist

Sample 11

−85

−69

−86

−94

−93

Negative

Sample 12

−83

−84

−91

−95

−88

Negative

Sample 13

487

−23

−78

394

−73

E.dispar

Sample 14

−75

−69

−81

−78

−71

Negative

Sample 15

530

−11

−65

478

−63

E.dispar

Average blank***

111

101

116

117

115

 
  1. *CTL= positive control from cloned-derived PCR biotinylated products.
  2. **Real time PCR specific for E. histolytica and E. dispar.
  3. *** From triplicate.