Figure 1
From: SvSXP: a Strongylus vulgaris antigen with potential for prepatent diagnosis
Silver stained gel. Lanes A+B illustrate that Strongylus vulgaris larval and adult excretory/secretory (ES) fractions contain a highly diverse mixture of molecules. Lane A: larval ES, lane B: adult ES – both stained by silver staining. To the left the low range molecular markers are shown. Western blot analysis: Lane C: reactivity of pre-immunisation rat serum against larval ES; lane D: reactivity of anti-adult S. vulgaris ES probed against larval ES; lane E: anti-adult S. vulgaris ES probed against adult ES; and lane F: reactivity of anti-adult S. vulgaris ES against larval ES. Pre-immunisation serum did not recognise molecules in either ES fraction (lanes C and F), and the hyperimmune serum recognised more molecules in adult ES (lane E) than in larval ES (lane D). To the right the low range molecular markers are shown.