Figure 5From: Characterization of TgPuf1, a member of the Puf family RNA-binding proteins from Toxoplasma gondiiRNA binding analysis of rTgPuf1 PUM-HD. EMSA titration of rTgPuf1 PUM-HD binding toPfs28 RNA1 (A), hb NRE RNA (B), and Pfs28 RNA1M (C). In Pfs28 RNA1M, the UGU sequence in the putative PBE of Pfs28 RNA1 was mutated to UCC. (D), (E) and (F) Quantitation of dissociation constant (Kd) values based on EMSA analysis from (A), (B) and (C), respectively.Back to article page