Cryogenic preservation of Dirofilaria immitis microfilariae, reactivation and completion of the life-cycle in the mosquito and vertebrate hosts

Table 2 Percentage survival and qualitative motility observed of Dirofilaria immitis microfilariae at 6 h and 3 and 5 days after thawing from a cryopreserved state of − 80 °C for > 30 days

Day post-thaw	Survival parameter	Cryoprotectant/cryoprotectant combination
Day post-thaw	Survival parameter	5% Glycerol	5% DMSO	6% DMSO	5% Glycerol/PVP^a	5% DMSO/PVP^a	6% DMSO/PVP^a
Day 0 (6 h)	Qualitative motility	Fast	Fast	Fast	Fast	Fast	Fast
Day 3	% Survival	36	69	63	61	93	88
Day 3	Qualitative motility	Fast	Fast	Fast	Fast	Fast	Fast
Day 5	% Survival	30	76	58	73	94	93
Day 5	Qualitative motility	Medium	Medium	Medium	Medium	Medium	Medium

ISSN: 1756-3305