Table 1 Primers used in Theileria equi genotype-specific polymerase chain reaction assays
Genotypes | Primers (5ʹ–3ʹ)a | Annealing temperature (°C) | Amplicon size (base pairs) | |
|---|---|---|---|---|
Forward | Reverse | |||
A | CGTTGCGGCTTGGTTGGGTTTCGATTA | GCAAAGTCCCTCTAAGAAGCGGA | 70 | 692 |
B | GTGGTCCTTCGCTATGTCGAGTGGTCC | CGCAAAGTCCCTCTAAGAAGCGATGGT | 66 | 718 |
C | TGTATCGTTATCTTCTGCTTGACAGTTTGG | GTCCCTCTAAGAAGCAGTGTAGAACATAAC | 66 | 714 |
D | TGTATCGTTTTCCTCTGCTTGACAGTTGGA | GTCCCTCTAAGAAGCAGTGTAGAACTAG | 64 | 719 |
E | ATCGTGGTTCTTCGCTATGTCGAGTGATCT | CGCAAAGTCCCTCTAAGAAGCGATAAC | 72 | 722 |