Fig. 7

Changes in pulmonary iron accumulation in the experimental MA-ALI mice upon GsMTx4 treatment. A Representative figures of pulmonary iron accumulation in mice across different groups using Perl’s Prussian blue staining under light microscope at 400 × magnification. Naive mice a; GsMTx4-treated uninfected mice b; Plasmodium berghei ANKA-infected control mice at 7 days p.i. c; GsMTx4-treated infected mice at 7 days p.i. d; P. berghei ANKA-infected control mice at 14 days p.i. e; GsMTx4-treated infected mice at 14 days p.i. f. B IOD/area of positive Perl’s Prussian blue stained cells was calculated in > 20 lung fields per animal (n = 4–5 mice/group). The differences in IOD/area between two groups and among multiple groups were analyzed by independent sample t-test and one-way ANOVA test, respectively. NS, P > 0.05 vs. naive mice; ^#P < 0.05 and ^##P < 0.01 vs. naive mice; ^&P < 0.05 and ^&&P < 0.01 vs. infected control mice at 7 days p.i.; ^*P < 0.05 and ^**P < 0.01 vs. infected control mice at 14 days p.i. Data are expressed as mean ± SD; the experiments were performed with four to five mice per group