Fig. 8

Changes in pulmonary Piezo1 and ferroptosis in the experimental MA-ALI mice upon GsMTx4 treatment. A–C Representative figures for pulmonary GPX4 (an inhibitor of ferroptosis), 4-HNE (an activator of ferroptosis) and Piezo1 in mice across different groups using immunohistochemical staining under a light microscope at 400 × and 200 × magnification. The positively stained GPX4, 4-HNE and Piezo1 cells are illustrated by dark-brown staining. Naive mice a; GsMTx4-treated uninfected mice b; Plasmodium berghei ANKA infected-control mice at 7 days p.i. c; GsMTx4-treated infected mice at 7 days p.i. d; P. berghei ANKA infected control mice at 14 days p.i. e; GsMTx4-treated infected mice at 14 days p.i. f. D IOD/area of positively stained GPX4, 4-HNE and Piezo1 cells was calculated in > 20 lung fields per animal (n = 4–5 mice/group). The differences in IOD/area between two groups and among multiple groups were analyzed by independent sample t-test and one-way ANOVA test, respectively. NS, P > 0.05 vs. naive mice; ^#P < 0.05 and ^##P < 0.01 vs. naive mice; ^&P < 0.05 and ^&&P < 0.01 vs. infected control mice at 7 days p.i.; ^*P < 0.05 and ^**P < 0.01 vs. infected control mice at 14 days p.i. Data are expressed as mean ± SD; the experiments were performed with four to five mice per group