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Fig. 4 | Parasites & Vectors

Fig. 4

From: Inhibition of Foxp3 expression in the placenta of mice infected intraperitoneally by toxoplasma gondii tachyzoites: insights into the PPARγ/miR-7b-5p/Sp1 signaling pathway

Fig. 4

T. gondii antigens elevated the miR-7b promoter activity. a Luciferase reporter assay was applied to detect the luciferase activity in EL4 cells that were electroporated with pGL3-E or pGL3-E-7b and then stimulated by ESA for 24 h, respectively. b Schematic diagram showed the truncation sequences at miR-7b promoter region. c The luciferase activities in EL4 cells, transfected respectively with recombinant plasmids, including pGL3-E, pGL3-E-7b, and pGL3-E-7b-A/B/C, were evaluated at 48 h post-transfection. d The luciferase activities in EL4 cells that were transfected respectively with recombinant plasmids including pGL3-E, pGL3-E-7b, and pGL3-E-7b-A/B/C, and then stimulated by ESA for 24 h and assayed by luciferase reporter assay at 48 h post-transfection. Data were representative of the results of three independent experiments (mean ± SD). Statistical analysis was conducted using one-way ANOVA Tukey’s multiple comparisons test (c) and two-way ANOVA with Sidak’s multiple comparisons test (a and d). *: P < 0.05. n.s.: P > 0.05

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Parasites & Vectors

ISSN: 1756-3305

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