Table 1 Primers used in this study
Primer | Sequence (5′ to 3′) |
|---|---|
For quantitative real-time PCR | |
mmu-miR-7b-5p | TGGAAGACTTGTGATTTTGTTGTT |
mmu-miR-16-5p | TAGCAGCACGTAAATATTGGCG |
mmu-miR-21a-3p | CAACAGCAGTCGATGGGCTGTC |
mmu-miR-27a-3p | TTCACAGTGGCTAAGTTCCGC |
mmu-miR-326-3p | CCTCTGGGCCCTTCCTCCAGT |
mmu-miR-149-3p | GAGGGAGGGACGGGGGCGGTGC |
mmu-miR-185-3p | AGGGGCTGGCTTTCCTCTGGT |
mmu-miR-338-3p | TCCAGCATCAGTGATTTTGTTG |
mmu-miR-448-3p | TTGCATATGTAGGATGTCCCAT CCCATCCCCAGGAGTCTTG ACCATGACTAGGGGCACTGTA |
Foxp3 F | |
Foxp3 R | |
GAPDH F | AGGTCGGTGTGAACGGATTTG |
GAPDH R | TGTAGACCATGTAGTTGAGGTCA |
For western blot | |
miR-7b mimic F | UGGAAGACUUGUGAUUUUGUUGUU |
miR-7b mimic R | CAACAAAAUCACAAGUCUUCCAUU |
miR-7b inhibitor F | AACAACAAAAUCACAAGUCUUCCA |
miR-16 mimic F | UAGCAGCACGUAAAUAUUGGCG |
miR-16 mimic R | CCAAUAUUUACGUGCUGCUAUU |
miR-16 inhibitor F | CGCCAAUAUUUACGUGCUGCUA |
NC mimic F | UUCUCCGAACGUGUCACGUTT |
NC mimic R | ACGUGACACGUUCGGAGAATT |
NC inhibitor F | CAGUACUUUUGUGUAGUACAA |
For plasmid construction | |
WT-Sp1 3′ UTR F | CCGCTCGAGCAACAGAGTGGAAAAGCCTGAAGTA |
WT-Sp1 3′ UTR R | ATAAGAATGCGGCCGCATTGTGTGCTTCCCTTTCCCTTAG |
MUT-Sp1 3′ UTR F | CGGATTTCAGTATCATTAAAAAAAAAATAGCTATAAGCCTCAATG |
MUT-Sp1 3′ UTR R | CATTGAGGCTTATAGCTATTTTTTTTTTAATGATACTGAAATCCG |
pGL3-E-7b F | TTACAAGCCTGAGTGTGTGTCCTCCCC |
pGL3-E-7b R | GCTAGACACCCTCTCCTGTCCG |
pGL3-E-7b-A F | ACTTCATCTTCCCTGTGTAGAATTG |
pGL3-E-7b-B F | CTGCAGCTGCTGGTGAGCAGGA |
pGL3-E-7b-C F | CTCAAGTTCTTTTTATTTTTAA |
pGL3-E-7b-A-Mut 1 F | GCCTTCCCCTTAAGCTTTTTTTTTTTTAACGTCGGAAACTGG |
pGL3-E-7b-A-Mut 1 R | CGGAAGGGGAATTCGAAAAAAAAAAAATTGCAGCCTTTGACC |
pGL3-E-7b-A-Mut 2 F | TGCCTGTGTCTCAGGTTTTTTTTTTTTAAAGGGATGTCTAGG |
pGL3-E-7b-A-Mut 2 R | ACGGACACAGAGTCCAAAAAAAAAAAATTTCCCTACAGATCC |