Fig. 5

A, B Treatment with two doses of fluralaner (Exzolt^®) showed higher insecticidal efficacy for Rhodnius prolixus, Triatoma infestans and Triatoma brasiliensis than a single-dose treatment. Chickens were treated with one or two doses of 0.5 mg/kg fluralaner (Exzolt^®), and mortality (%) of Rhodnius prolixus (A), Triatoma infestans (B), Triatoma brasiliensis (C) and Triatoma pseudomaculata (D) was assessed before treatment and 1, 7, 14, 21, 28, 35 and 56 days after treatment. Ten nymphs of the 3rd, 4th and 5th instar were allowed to feed on each chicken at each time point. Data are shown as means, and a generalized linear mixed model (GLMM) was conducted to compared mortality among groups. Mortality curves with different lowercase letters are significantly different at P < 0.05. Closed symbols represent insects that fed on fluralaner-treated chickens, open symbols represent insects that fed on untreated control chickens