DNA vaccination with a gene encoding Toxoplasma gondii GRA6 induces partial protection against toxoplasmosis in BALB/c mice
© Sun et al; licensee BioMed Central Ltd. 2011
Received: 24 September 2011
Accepted: 9 November 2011
Published: 9 November 2011
Infection with the protozoan Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. Protection from acute toxoplasmosis is known to be mediated by CD8+ T cells, but the T. gondii antigens and host genes required for eliciting protective immunity have been poorly defined. The T. gondii dense granule protein 6 (GRA6), recently proved to be highly immunogenic and produces fully immune protection in T. gondii infected BALB/c mice with an H-2Ld gene. The CD8+ T cell response of H-2Ld mice infected by the T. gondii strain seemed to target entirely to a single GRA6 peptide HF10-H-2Ld complex.
To determine whether a GRA6-based DNA vaccine can elicit protective immune responses to T. gondii in BALB/c mice, we constructed a eukaryotic expression vector pcDNA3.1-HisGRA6 and tested its immunogenicity in a mouse model. BALB/c mice were vaccinated intramuscularly with three doses of GRA6 DNA and then challenged with a lethal dose of T. gondii RH strain tachyzoites. All immunized mice developed high levels of serum anti-GRA6 IgG antibodies, and in vitro splenocyte proliferation was strongly enhanced in mice adjuvanted with levamisole (LMS). Immunization with pcDNA3.1-HisGRA6 with LMS resulted in 53.3% survival of challenged BALB/c mice as compared to 40% survival of BALB/c without LMS. Additionally, immunized Kunming mice without an allele of H-2Ld failed to survive.
Our result supports the concept that the acquired immune response is MHC restricted. This study has a major implication for vaccine designs using a single antigen in a population with diverse MHC class I alleles.
Infection with the intracellular parasite Toxoplasma gondii is responsible for toxoplasmosis in humans and other warm-blooded animals. In veterinary medicine, T. gondii infection has economic importance due to abortion and neonatal losses in domestic animals, or as a source of transmission to humans [1–3]. Vaccination is one of the most efficient strategies to prevent and control the spread of toxoplasmosis. A live attenuated vaccine has been developed for the prevention of chronic infection in sheep . However, it cannot be used in humans because of the risk of reversion to a pathogenic form .
The dense granule of T. gondii is a secretory vesicular organelle, which produces proteins that participate in the modification of the parasitophorous vacuole (PV) and PV membrane for the maintenance of intracellular parasitism in almost all nucleated host cells . There are 16 GRA proteins, GRA1-GRA10, GRA12, GRA14, 2 isoforms of nucleotide triphosphate hydrolase (NTPase I and II)  and 2 protease inhibitors (TgPI 1 and 2) [8, 9]. All the GRA proteins are identified as excretory/secretory antigens (ESP). Several of them are suitable as DNA vaccines for immunity against toxoplasmosis. Immunization of C3H mice with a plasmid expressing granule protein 1 (GRA1) showed 75-100% protection to challenge with T. gondii cysts . DNA vaccination with protein GRA1, GRA7, and rhoptry protein ROP2 induced protection against infection with different virulent T. gondii strains in C3H mice but not in BALB/c and C57BL/6 mice . The GRA4 DNA vaccine containing the whole coding sequence, results in a 62% survival of susceptible C57BL/6 infected mice . Intramuscular injection of sheep with a DNA liposome formulated plasmid coding for GRA1, GRA4, GRA6 and GRA7 is an effective system that induces a significant immune response against T. gondii.
Protection from acute toxoplasmosis is mediated by CD8+ T cells, but T. gondii antigens and host genes required for eliciting protective immunity are poorly defined . The T. gondii dense granule protein 6 (GRA6), being highly immunogenic, is a candidate vaccine against toxoplasmosis. The HF10 peptide (HPGSVNEFDF) is located at the carboxyl terminus of GRA6 and is the immunodominant epitope to bind H-2Ld major histocompatibility complex class I molecule (MHC class I). It induces immune protection of BALB/c mice carrying the H-2Ld molecule against T. gondii infection. The CD8+ T cell response of BALB/c mice infected by the T. gondii strain seemed to be targeted entirely to the single GRA6 HF-H-2Ld complex . Notably, similar focusing of the CD8+ T cell response to a single antigen from the circumsporozoite protein of Plasmodium yoelii and only a small subset of epitopes of the trans-sialidase antigens of Trypanosoma cruzi has been reported in mice [16, 17]. DNA-based vaccination is one of the most promising strategies for the development of new generation effective vaccines against intracellular parasites.
We have constructed the eukaryotic expression vector named pcDNA3.1-HisGRA6 to determine whether DNA immunization can elicit protective immune responses to T. gondii. The present work shows that the partial protection against toxoplasmosis in BALB/c mice induced by DNA vaccination with T. gondii GRA6 gene. It has implications for vaccine designs using a single antigen in a population with diverse MHC class I alleles.
Materials and methods
Plasmid construction and preparation
The entire GRA6 open reading frame (ORF) was amplified by PCR from the cDNA of T. gondii (RH strain) tachyzoites with primer RH-1 (5'-CGCGGATCCCGCCAGCATGGCACACGGTGGCATC-3') and RH-2 (5'-GCATGCGGCCGCCTAATAATCAAACACATTCACACG-3'). The PCR product was ligated into the pGEX-4T-1 and pcDNA3.1 vectors (Invitrogen) at the BamH I and Not I sites to construct two recombinant plasmids pGEX-GRA6 and pcDNA3.1-HisGRA6. The two plasmids pGEX-GRA6 and pcDNA3.1-HisGRA6, carrying the GRA6 gene were purified from DH5α-competent cells with an endotoxin-free DNA purification kit (Vigorous Biotechnology, Beijing). The integrity of these inserts was verified by DNA sequencing and agarose gel electrophoresis after digestion with appropriate restriction enzymes. The control plasmid pcDNA3.1 was purified from the same E. coli strain using the same method as purifying pcDNA3.1-HisGRA6. Plasmid pGEX-GRA6 was used for expressing the GRA6 protein in bacteria and plasmid pcDNA3.1-HisGRA6 was used for immunization of mice and protein expression in mammalian cells in vitro. DNA for immunization was dissolved in sterile endotoxin-free PBS. The DNA concentration was determined spectrometrically at 260 nm. The OD 260/280 ratios for the purified DNA were 1.8-2.0, indicating that there was no major protein contamination.
Mice and parasites
Six- to eight-week-old female BALB/c carrying H-2Ld and Kunming (without H-2Ld) mice were purchased from the Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences, Beijing. They were maintained in groups of six per cage with food and water provided ad libitum and artificial light for 12 h per day. This study was conducted in compliance with the regulations concerning the use of laboratory animals of China Agricultural University. The RH strain of T. gondii was maintained in our laboratory by passage of tachyzoites in BALB/c mice or Vero cells. The T. gondii tachyzoites used for challenge were cultured in Vero cells in DMEM medium (Sigma, USA) supplemented with 2% fetal calf serum, harvested by scrapping the cells and purified by filtration on glass fiber .
Expression of GRA6 in Chinese hamster ovary (CHO) cells
The plasmid pcDNA3.1-HisGRA6 or the control plasmid pcDNA3.1 were transfected into CHO cells using a commercial transfection kit (Engreen, Beijing) according to the manufacturer's instructions. CHO cells were cultured in DMEM supplemented with FBS (10% v/v), penicillin (200 U ml-1) and streptomycin (20 mg ml-1) in a humidified atmosphere of 5% CO2 at 37°C. The GRA6 mRNA transcription and protein expression in CHO cells were confirmed by RT-PCR and western blotting, respectively. The transfected CHO cells were processed for identification of the GRA6 mRNA transcription by RT-PCR and GRA6 protein expression by immunoblotting according to the standard technique . In brief, cells were harvested 48 h after transfection, disrupted by rapid freezing and thawing 3 times using liquid nitrogen, the supernatant was obtained by centrifuging at 5, 000 g for 15 min at 4°C. Proteins in the supernatant of the samples were separated by SDS-PAGE and blotted onto a PVDF membrane, which was then blocked with 5% skimmed milk. The PVDF membrane was incubated for 1 h with murine anti-His antibodies (Tiangen, Beijing) diluted 1:4000 in 5% skimmed milk, and incubated with HRP-conjugated anti-mouse IgG (Southern Biotech, USA) diluted 1:8000 in 5% skimmed milk. The conjugated substrate was detected using a chemiluminescent dectection kit (CWBIO, China).
Expression of GST-GRA6 protein in bacteria
The plasmid pGEX-GRA6 was transformed to E. coli BL21 (DE3) cells (Transgen Company, China) with kanamycin selection. The recombinants were harvested after 6 h of induction with IPTG (isopropyl β-D-1-Thiogalactopyranoside). The glutathione-S-transferase (GST)-GRA6 protein was purified using glutathione affinity chromatography.
Antigen-specific antibodies were quantified by enzyme-linked immuno-sorbent assay (ELISA) as previously described . GST-GRA6 protein expressed in bacteria was used as the coating antigen, and antigen-specific antibodies were detected with HRP-labeled anti-mice IgG. The assay was developed using TMB. The optical density (OD) at 450 and 630 nm was measured by a plate reader (Bio-Rad, CA, USA). The serum antibody titer was defined as the highest dilution that gave a test/naive serum OD ratio of 2.1 or higher.
Antigen-specific splenic lymphocyte proliferation
GRA6-stimulated splenic lymphocyte proliferation was used to assess the cellular immune response of the immunized mice. Three mice from each group were killed 7 days after the last immunization and the spleen was removed. Single cell suspensions were obtained by filtration through a nylon mesh. Erythrocytes in the spleen cell suspension were removed by lysis and the remaining cells were washed and suspended in RPMI 1640 medium (GIBCO) supplemented with 5% FCS, HEPES (10 mM), L-glutamine (2 mM), sodium pyruvate (1 mM), β-mercaptoethanol (50 mM), gentamycin (50 mg/ml), penicillin (100 U/ml) and streptomycin (100 mg/ml). They were seeded in triplicate in flat-bottomed 96-well microtiter plates (Costar) at 5 × 105 cells per well in 200 μl culture medium alone as the negative control or with various concentrations of GST-GRA6 fusion protein, bovine serum albumin (BSA), or 5 μg/ml concanavalin A as the positive control for 48-72 h at 37°C. Then 20 μl MTT (5 mg/ml in PBS) was added to each well. After 4 h of incubation at 41°C DMSO (100 μl/well) was added and the culture was further incubated at 37°C for 10 min to arrest cell proliferation. The optical density was measured by a plate reader at 490 nm. Data were expressed as stimulation index (SI), which is the ratio of the mean OD value of triplicate wells with antigen stimulation to the mean value of triplicate wells of the negative control .
The formalin-fixed brain, liver and spleen tissues were dehydrated with increasing concentrations of ethanol, embedded in paraffin, sectioned and stained with hematoxylin and eosin for histological evaluation.
Levels of significance between groups of mice were determined using the student's t-test for mortality, antibody titers and T cell proliferation stimulation index.
Construction of DNA vaccine
Expression of GRA6 in vitro
The histidine tagged GRA6 protein of 32 kDa was identified by Western blotting using anti-His monoclonal antibodies (Figure 3B).
Humoral immune response induced by DNA vaccination
Cellular immune response induced by DNA vaccination
Immunoprotection against lethal challenge
To determine whether the DNA vaccine could induce immunoprotection against a lethal challenge of T. gondii, BALB/c mice were immunized with pcDNA3.1-HisGRA6 alone, or pcDNA3.1-HisGRA6 plus LMS and challenged with T. gondii tachyzoites.
In the present study, a DNA vaccine was constructed based on the HF10 peptide sequence located at the carboxyl terminus of the GRA6 gene of T. gondii. The results from pcDNA3.1-HisGRA6-transfected CHO cells show that the recombinant GRA6 can be expressed in eukaryotic cells in vitro. Our data demonstrated that a single epitope DNA vaccine, pcDNA3.1-HisGRA6 was able to induce humoral and cellular immunity in mice and confer partial protection against acute infection of T. gondii. We also showed that immunogenicity of the DNA vaccine was enhanced by a non-specific chemical adjuvant, LMS. This is the first study demonstrating that a single epitope based DNA vaccine induced protective immunity against the acute T. gondii infection.
The immune response to T. gondii infection is dominated by CD8+ T cells, which is critical in the protection of hosts from intracellular parasite infection [22, 23]. Resistance to toxoplasmic encephalitis has been linked to the locus encoding H-2Ld MHC class I in mice carrying the H-2Ld gene . Blanchard et al.  found that the GRA6 protein, a polymorphic protein secreted in the parasitophorous vacuole, is an immunodominant antigen and elicited a protective CD8+ response in mice through the presentation of a single decapeptide HF10 of GRA6 by the H-2Ld major histocompatibility complex class I molecule. Based on the above findings, we designed a DNA vaccine expressing the HF10 fragment of GRA6, which was expected to induce strong cellular immunity and confer good protective efficacy against toxoplasmosis in BALB/c mice carrying the H-2Ld gene but not in Kunming mice without the H-2Ld. Indeed, two injections of the DNA vaccine to BALB/c mice induced strong GRA6-specific cellular and humoral immunity as shown by a significant increase in serum antibodies to GRA6 and splenic lymphocyte expansion specific to GRA6 in vitro.
The use of adjuvants to improve vaccine efficacy is well known, and adjuvant is used in many commercial vaccines for humans and animal species. The function of adjuvants is believed to activate innate immunity, to increase the interaction between antigen and antigen presenting cells (APC) and to improve antigen processing or presentation in APC . In this study, we used a chemical adjuvant, LMS, which is quickly absorbed or metabolized by tissues at the injection site. LMS has been commonly used as an anthelmintic for domestic animals for more than 30 years and also used in several human clinical trials as an anticancer drug . LMS injected with a Foot-and-Mouth Disease Virus (FMDV) DNA vaccine stimulated both humoral and cellular immune responses in conjunction with strong production of interferon (IFN)-γ . We also observed some enhancement in our study, which may relate to LMS affecting several important genes within the toll-like pathway . LMS might be an agonist that could activate innate response via TLR7/8 pathways. Indeed, CpG motifs [30, 31], derived from the eukaryotic vector, activated innate responses via the TLR9 pathway.
The DNA vaccine based on GRA6 of T. gondii can induce strong humoral and cellular immunity and provide partial protection against toxoplasmosis in BALB/c mice with an H-2Ld allele. Our result supports the notion that the immune response to T. gondii is MHC restricted. This study has major implications for vaccine designs using a single antigen in a population with diverse MHC class I alleles . It provides a theoretical basis for the vaccine design using a single immunodominant antigen for other pathogens for which T cells are required for host protection.
This work was supported by the National S & T Major Program (Grant No. 2008ZX10004-011).
- Dubey JP: Status of toxoplasmosis in sheep and goats in the United States. J Am Vet Med Assoc. 1990, 196: 259-262.PubMedGoogle Scholar
- Dubey JP, Scandrett WB, Kwok OC, Gajadhar AA: Prevalence of antibodies to Toxoplasma gondii in ostriches (Struthio camelus). J Parasitol. 2000, 86 (3): 623-624.View ArticlePubMedGoogle Scholar
- Remington JS, McLeod R, Desmonts G: Toxoplasmosis. Infectious diseases of the fetus and newborn infant. Edited by: Remington JS, Klein JO. 1995, W.B. Saunders. Philadelphia, 140-267. 4Google Scholar
- Buxton D: Toxoplasmosis: the first commercial vaccine. Parasitol Today. 1993, 9: 335-337.View ArticlePubMedGoogle Scholar
- Ogra PL, Faden HS, Abraham R, Dubey LC, Sun M, Minor PD: Effect of prior immunity on the shedding of virulent revertant virus in feces after oral immnunization with live attenuated poliovirus vaccines. J Infect Dis. 1991, 164: 191-194.View ArticlePubMedGoogle Scholar
- Nam HW: GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane. Korean J Parasitol. 2009, 47: S29-S37.PubMed CentralView ArticlePubMedGoogle Scholar
- Johnson M, Broady K, Angelici MC, Johnson A: The relationship between nucleotide triphosphate hydrolase (NTPase) isoform and Toxoplasma strain virulence in rat and human toxoplasmosis. Microbes Infect. 2003, 5: 797-806.View ArticlePubMedGoogle Scholar
- Morris MT, Coppin A, Tomavo S, Carruthers VB: Functional analysis of Toxoplasma gondii protease inhibitor I. J Biol Chem. 2002, 227: 45259-45266.View ArticleGoogle Scholar
- Pszenny V, Ledesma BE, Matrajt M, Duschak VG, Bontempi EJ, Dubremetz JF, Angel SO: Subcellular localization and post-secretory targeting of TgPI, a serine proteinase inhibitor from Toxoplasma gondii. Mol Biochem Parasitol. 2002, 121: 283-286.View ArticlePubMedGoogle Scholar
- Scorza T, D'Souza S, Laloup M, Dewit J, De Braekeleer J, Verschueren H, Vercammen M, Huygen K, Jongert E: A GRA1 DNA vaccine primes cytolytic CD8(+) T cells to control acute Toxoplasma gondii infection. Infect Immun. 2003, 71: 309-316.PubMed CentralView ArticlePubMedGoogle Scholar
- Vercammen M, Scorza T, Huygen K, De Braekeleer J, Diet R, Jacobs D: DNA vaccination with genes encoding Toxoplasma gondii antigens GRA1, GRA7, and ROP2 induces partially protective immunity againstblethal challenge in mice. Infect Immun. 2000, 68 (1): 38-45.PubMed CentralView ArticlePubMedGoogle Scholar
- Desolme B, Mevelec MN, Buzoni-Gatel D, Bout D: Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene. Vaccine. 2000, 18: 2512-2521.View ArticlePubMedGoogle Scholar
- Hiszczyn'ska-Sawicka E, Ole˛dzka G, Holec-Ga˛sior L, Li H, Xu JB, Sedcole R, Kur J, Bickerstaffe R, Stankiewicz M: Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii. Vet Parasit. 2011, 177: 281-289.View ArticleGoogle Scholar
- Denkers EY, Gazzinelli RT: Regulation and function of T cell mediated immunity during T. gondii infection. Clin Microbiol Res. 1998, 11: 569-588.Google Scholar
- Blanchard Nicolas, Gonzalez Federico: Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum. Nat Immunol. 2008, 9: 937-944.View ArticlePubMedGoogle Scholar
- Kumar KA, Sano G, Boscardin S, Nussenzweig RS, Nussenzweig MC, Zavala F, Nussenzweig V: The circumsporozoite protein is an immunodominant protective antigen in irradiated sporozoites. Nature. 2006, 444: 937-940.View ArticlePubMedGoogle Scholar
- Martin DL, Weatherly DB, Laucella SA, Cabinian MA, Crim MT, Sullivan S, Heiges Mark, Craven SH, Rosenberg CS, Collins MH, Sette A, Postan M, Tarleton RL: CD8+ T-Cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes. PLoS Pathog. 2006, 2: e77-PubMed CentralView ArticlePubMedGoogle Scholar
- Grimwood BG, Hechemy K, Stevens RW: T. gondii: purification of trophozoites propagated in cell culture. Exp Parasitol. 1979, 48: 282-286.View ArticlePubMedGoogle Scholar
- Harning D, Spenter J, Metsis A, Vuust J, Petersen E: Recombinant Toxoplasma gondii SAG1 (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific IgM and IgG antibodies. Clin Diagn Lab Immunol. 1996, 3: 355-357.PubMed CentralPubMedGoogle Scholar
- BenoõÃt D, Marie-NoeÈlle M, Dominique BG, Daniel B: Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene. Vaccine. 2000, 18: 2512-2521.View ArticleGoogle Scholar
- Wagner G, Card J, Cowan K: Immunochemical studies of foot-and-mouth disease. VII. Characterisation of foot-and mouth disease virus concentrated by polyethylene glycol precipitation. Arch Ges Virusforsch. 1990, 30: 343-352.View ArticleGoogle Scholar
- Suzuki Y, Remington JS: Dual regulation of resistance against Toxoplasma gondii infection by Lyt-2+ and Lyt-1+, L3T4+ T cells in mice. J Immunol. 1988, 140: 3943-3946.PubMedGoogle Scholar
- Parker SJ, Roberts CW, Alexander J: CD8+ T cells are the major lymphocyte subpopulation involved in the protective immune response to Toxoplasma gondii in mice. Clin Exp Immunol. 1991, 84: 207-212.PubMed CentralView ArticlePubMedGoogle Scholar
- Brown CR: Definitive identification of a gene that confers resistance against Toxoplasma cyst burden and encephalitis. Immunol. 1995, 85: 419-428.Google Scholar
- Gajria B: ToxoDB: an integrated Toxoplasma gondii database resource. Nucleic Acids Res. 2008, 36: 553-556.View ArticleGoogle Scholar
- Gupta RK, Siber GR: Adjuvants for human vaccines--current status, problemsand future prospects. Vaccine. 1995, 13: 1263-1276.View ArticlePubMedGoogle Scholar
- Laurie JA, Moertel CH, Fleming TR: Surgical adjuvant therapy of large-bowel carcinoma: An evaluation of levamisole and the combination of levamisole and fluorouracil. J Clin Oncol. 1989, 7: 1447-1459.PubMedGoogle Scholar
- Jin H, Li Y, Ma Z, Zhang F, Xie Q, Gu D, Wang B: Effect of chemical adjuvants on DNA vaccination. Vaccine. 2004, 22: 2925-2935.View ArticlePubMedGoogle Scholar
- Pietras EM, Saha SK, Cheng G: The interferon response to bacterial and viral infections. J Endotoxin Res. 2006, 12 (4): 246-250.View ArticlePubMedGoogle Scholar
- Weeratna R, McCluskie M, Xu Y, Davis H: CpG DNA induces stronger immune responses with less toxicity than other adjuvants. Vaccine. 2000, 18: 1755-1762.View ArticlePubMedGoogle Scholar
- Johes TR, Obaldia NG, Gramzinki RA: Synthetic oligodeoxynucleotides containing CpG motifs enhance immunogenicity of a peptide malaria vaccine in Aotus monkeys. Vaccine. 1999, 17: 3065-3071.View ArticleGoogle Scholar
- Chung C, Mealey RH, McGuire TC: CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins. Virology. 2004, 327: 144-154.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.