The abundance and host-seeking behavior of culicine species (Diptera: Culicidae) and Anopheles sinensis in Yongcheng city, people's Republic of China
- Xiao-Bo Liu†1,
- Qi-Yong Liu†1Email author,
- Yu-Hong Guo†1,
- Jing-Yi Jiang2,
- Dong-Sheng Ren1,
- Guang-Chao Zhou2,
- Can-Jun Zheng4,
- Yan Zhang1,
- Jing-Li Liu1,
- Zhi-Fang Li1,
- Yun Chen1,
- Hong-Sheng Li2,
- Lindsay C Morton3,
- Hua-Zhong Li4,
- Qun Li4 and
- Wei-Dong Gu5, 6
© Liu et al; licensee BioMed Central Ltd. 2011
Received: 29 October 2011
Accepted: 24 November 2011
Published: 24 November 2011
The knowledge of mosquito species diversity and the level of anthropophily exhibited by each species in a region are of great importance to the integrated vector control. Culicine species are the primary vectors of Japanese encephalitis (JE) virus and filariasis in China. Anopheles sinensis plays a major role in the maintenance of Plasmodium vivax malaria transmission in China. The goal of this study was to compare the abundance and host-seeking behavior of culicine species and An. sinensis in Yongcheng city, a representative region of P. vivax malaria. Specifically, we wished to determine the relative attractiveness of different animal baits versus human bait to culicine species and An. sinensis.
Culex tritaeniorhynchus was the most prevalent mosquito species and An. sinensis was the sole potential vector of P. vivax malaria in Yongcheng city. There were significant differences (P < 0.01) in the abundance of both An. sinensis and Cx. tritaeniorhynchus collected in distinct baited traps. The relative attractiveness of animal versus human bait was similar towards both An. sinensis and Cx. tritaeniorhynchus. The ranking derived from the mean number of mosquitoes per bait indicated that pigs, goats and calves frequently attracted more mosquitoes than the other hosts tested (dogs, humans, and chickens). These trends were similar across all capture nights at three distinct villages. The human blood index (HBI) of female An. sinensis was 2.94% when computed with mixed meals while 3.70% computed with only the single meal. 19:00~21:00 was the primary peak of host-seeking female An. sinensis while 4:00~5:00 was the smaller peak at night. There was significant correlation between the density of female An. sinensis and the average relative humidity (P < 0.05) in Wangshanzhuang village.
Pigs, goats and calves were more attractive to An. sinensis and Cx. tritaeniorhynchus than dogs, humans, and chickens. Female An. sinensis host-seeking activity mainly occurred from 19:00 to 21:00. Thus, we propose that future vector control against An. sinensis and Cx. tritaeniorhynchus in the areas along the Huang-Huai River of central China should target the interface of human activity with domestic animals and adopt before human hosts go to bed at night.
KeywordsHost-seeking behavior mosquito culicine species Anopheles vectors ecology malaria elimination
Malaria remains a serious global public health threat and causes substantial morbidity and mortality in the world. The current estimate of human lives at risk from Plasmodium vivax malaria is 2.6 billion [1, 2] and South and East Asia account for 52% of the total P. vivax malaria burden . The estimated global cost of P. vivax malaria, including lost productivity, cost of health care, and transport to clinics, is between U.S. $1.4 and $4 billion per year . The interruption of P. vivax malaria transmission worldwide is still one of the greatest challenges for international health and development communities . Despite significant reductions in the overall burden of malaria in the 20th century [5–7], this parasitic disease still represents a major public health problem in China [6, 8], with dramatic re-emergence in the Huang-Huai River region of central China in 2001 . In Henan Province [9–13] and Anhui Province [14, 15], large-scale epidemics in recent years have caused a major public health concern. This situation not only has an impact on the region's economic development and people's living standards, but also poses a challenge for the routine malaria control strategy. In response to the global initiative to eradicate malaria [16, 17], an action plan for malaria elimination was proposed by the Chinese Ministry of Health in 2009 and a national elimination campaign was launched by the Chinese Government in 2010, to eliminate malaria in most endemic regions by 2015 and to achieve ultimate national elimination by 2020 .
Anopheles sinensis, Anopheles lesteri[19, 20], Anopheles minimus and Anopheles dirus are considered to be four important vector species of malaria in China [21, 22]. However, malaria outbreaks and re-emergences were only in areas with An. sinensis in recent years . In studies conducted in the areas along the Huang and Huaihe River, An. sinensis plays an important role in the maintenance of P. vivax malaria transmission [23, 24]. Yongcheng city, as a re-emergence region of P. vivax malaria in Henan Province, is one of the major malaria epidemic areas . After extensive control efforts, malaria was nearly eliminated in the 1990s [9, 25]; however, epidemics resurged and maintained in the area in the 2000s with the highest cases of 2,890 in 2006 [25, 26]. According to surveillance data of Yongcheng Center for Disease Control and Prevention (Yongcheng CDC), a decline in the malaria incidence rate was observed . Based on a study conducted by Zhou et al in 2010, An. sinensis was considered to be the sole potential vector of P. vivax malaria in Yongcheng city [5, 25, 27]. The estimated vectorial capacity of An. sinensis population in this area was 2.78 times higher in 2010 (0.4983) than in the 1990s . The epidemiological consequences of this change are unknown, but this observed entomological change cannot be ignored when transitioning from malaria control to elimination in China. In recent years, several factors, such as the adjustment of regional agricultural structures and the reduction of biological barriers (calf, pig, etc.), are likely to increase human-vector contact and increase the transmission rate of P. vivax malaria .
Vector abundance, host-seeking behavior and preference  are all important components of disease-transmission cycles [29, 30]. Host-seeking behavior has been operationally defined as the in-flight orientation of the avid female toward a potential blood meal host . Host-seeking typically commenced shortly after sunset and usually peaks during the succeeding one to three hours, the hottest and driest period at night [32, 33]. The host-seeking strategies used by arthropod vectors can, in part, affect the efficiency of disease transmission [30, 34]. Mosquitoes which prefer to feed on animals are less important in transmitting human disease than those which prefer to feed on humans . In particular, the knowledge of vector abundance and host-seeking activities is of critical importance for integrated vector control. Culex tritaeniorhynchus is the primary vector of the Japanese encephalitis (JE) virus [36, 37], and Culex pipiens pallens is the primary vector of the JE virus and filariasis in China. The host-seeking behavior of culicine species is of great significance in the epidemiology of the JE virus and filariasis and the host-seeking behavior of Anopheline mosquitoes is of great significance in the epidemiology of malaria [38, 39]. The host-seeking behavior can be studied by a variety of methods [40, 41], such as human landing catch (HLC) , human bait indoors and outdoors [38, 43], indoor residual spray (IRS), baited mosquito nets, baited resting boxes , light traps [45, 46], etc. Several approaches including serologic techniques, enzyme-linked immunosorbent assay (ELISA) and DNA-based techniques, have been applied to study the blood-feeding behavior of mosquitoes, black flies, ticks and other blood-feeding arthropods as it relates to host-parasite interactions and pathogen transmission [47–58].
Since culicine species and An. sinensis play a distinct role in disease transmission, the abundance and host-seeking behavior of culicine species and An. sinensis are of fundamental epidemiological importance , however, little information is available regarding species composition, abundance, host-seeking behavior, and the level of anthropophily exhibited by each species in Yongcheng city. The goals of this study were to compare the abundance and host-seeking behavior of culicine species and An. sinensis in villages of Yongcheng city characterized by different levels of historical incidence of P. vivax malaria and determine the relative attractiveness of different animal baits versus human bait to culicine species and An. sinensis. Such information is required in order to understand the comprehensive vector biology of culicine species and An. sinensis, for the design of effective vector control strategies and for implementation of the ongoing malaria elimination campaign in China.
Besides the difference in the levels of historical incidence of P. vivax malaria, other characteristic differences between the three studied villages are as follows: First, Dingtang village is adjacent to Guoyang County, and Renhu village is adjacent to Suixi County. Guoyang and Suixi County are unstable regions of P. vivax malaria in Anhui Province while Wangshanzhuang village is not adjacent to Anhui Province. Second, the water-body distributions and appropriate breeding habitats of culicine species and An. sinensis larvae in Dingtang village and Renhu village are more than those in Wangshanzhuang village. During the study period, there were many ponds and canals that contained endogenous lotus, and a large number of Anopheline mosquitoes and culicine species larvae were observed in Renhu village and Dingtang village. Third, the number of animal hosts in Renhu village and Dingtang village was larger than that of Wangshanzhuang village. Based on the investigative data of the village doctors, Dingtang village covers approximately 3,500 acres of arable land, with a population of 1,890 people, 1 calf, and 150 goats within 480 households, Renhu village covers approximately 4,500 acres of arable land, with a population of 1,875 people, 60 calves, 70 pigs, and 180 goats within 470 households, Wangshanzhuang village covers approximately 1,600 acres of arable land, with a population of 750 people, and 5 calves, 45 pigs, and 70 goats within 170 households, respectively.
Host-seeking behavior experiments
Host-seeking behavior experiments of culicine species and An. sinensis were conducted from August 28th to October 5th. The reason that these experiments were conducted during this period is that this period was considered to be the epidemic episode of P. vivax malaria along the Huaihe River region in China . Three villages were sampled in different weeks because of limited man power and material resources. Outdoor bednet traps [34, 60] were employed to study the host-seeking activity of culicine species and An. sinensis. The size of the bednet traps was 3.6 m × 2.4 m × 1.6 m. There was twenty centimeters between the floor and the bottom of the bednet traps. The locations of the bednet traps were close to ponds, ditches and drains, in a line or ellipse, at intervals of 50 m. The distance from the bednet traps to the nearest resident's house was about 60 m, and people living in the range of this distance near to the water-bodies had higher risk of malaria infection than those living beyond the distance . In each sampled village, major local domestic animals, such as calves, pigs, goats, dogs, and chickens, were used as animal baits to attract mosquitoes to the bednet traps. Some members of staff at Yongcheng CDC and China CDC were selected as human baits. These members (under double bednet traps to avoid mosquitoes bites) were also used repeatedly throughout the entire duration of the study to avoid bias [35, 45].
To control for the plausible confounding bias of locations of the bednet traps and collection nights, one 5 × 5 Latin square design and two 6 × 6 Latin square design experiments [34, 46, 61] were conducted. The Latin square design is used where researchers desire to control the variation in an experiment that is related to rows and columns in the field. Treatments (baits) were assigned at random within rows (nights) and columns (locations of the bednet trap), with each treatment once per row and once per column . During this period, three independent experiments were conducted, with one experiment per village. A 5 × 5 Latin square design experiment was conducted in Dingtang village because five types of bait, namely calves, goats, dogs, chickens and humans, existed in this village while two 6 × 6 Latin square design experiments were conducted in Renhu village and Wangshanzhuang village because six types of bait, namely calves, pigs, goats, dogs, chickens, and humans existed in these two villages.
Data was recorded for collection dates and sites, sequences of locations of the bednet traps, collection nights, and numbers of each mosquito species. Temperature (°C) and relative humidity (%) were the average value from a weather web in China http://www.weather.com.cn. Ambient outdoor air temperature and relative humidity of each collection hour was recorded using a WS-1 Thermo-Hygrometer device. Since some events such as heavy rains would prevent host-seeking activity, mosquito sampling occasions were scheduled based on the pattern of weather conditions. During the trapping days, however, rainfall values (mean minimum and maximum in mm) were obtained from a local meteorological station located in Yongcheng city, so that the effect of this environmental factor could be evaluated while controlling for between-night variation in mosquito abundance.
Blood meal identification
Primer sequences for the cytochrome b-based polymerase chain reaction blood meal identification assay
Product size with
GGC TTA CTT CTC TTC ATT CTC TCC T
CAT CGG CAC AAA TTT AGT CG
CCT CGC AGC CGT ACA TCT C
CCT AAT CTT AGT ACT TGT ACC CTT CCT C
GGA ATT GTA CTA TTA TTC GCA ACC AT
GGT TGT CCT CCA ATT CAT GTT A
The species composition of mosquitoes in the three villages was calculated. Abundance (mean catch per bait per night) of culicine species and An. sinensis were calculated for each site and bait. The number of culicine species and An. sinensis of each bait was log (x+1) transformed and subjected to variance analysis after a satisfactory check for normality of the distribution. Univariate analysis of variance (general linear models functions) was used to test the main effect and interaction effects of bait types, collection nights, and locations of the bednet traps. Probabilities of the Univariate analysis of variance were at α = 0.05 level. Pair wise comparison had been performed to rank host-seeking behavior of culicine species and An. sinensis. Normal distributions of the An. sinensis density, the average temperature, and the average relative humidity were tested by One-sample of Kolmogorove-Smirnov Test before using Pearson correlation analysis. The association between the density of An. sinensis and the average temperature, between the density of An. sinensis and the average relative humidity were examined by Pearson correlation analysis. Data was entered and analyzed by SPSS (Statistical Package for the Social Sciences) statistical software (version 11.5).
Ethical approval for this study was obtained from the Ethical Committee of China CDC and permission was also obtained from the Municipal Government, the Municipal Health Bureau and CDC in Yongcheng city.
A total of 35,312 mosquitoes were captured in the three villages during the study period, of which 76.6% (n = 27,048) were Cx. tritaeniorhynchus, 12.2% (n = 4,313) were Cx. pipiens pallens, 10.6% (n = 3,755) were An. sinensis, 0.02% (n = 9) were Aedes albopictus, 0.5% (n = 165) were Armigeres subalbatus (Coquillett), and 0.1% (n = 22) were others. Cx. tritaeniorhynchus was the most prevalent mosquito species, and An. sinensis was the sole potential vector of P. vivax malaria in Yongcheng city. As far as sex proportions of captured An. sinensis is concerned, 1.25% (n = 47) out of 3, 755 An. sinensis were males, while 98.75% (n = 3,708) were females. Fifty percent (n = 102) of blood-fed female Anopheline mosquitoes, which were sampled, about the same proportions from the bednet traps in the three villages, were further identified to species by ribosomal DNA PCR assay. All collected adult female Anopheline mosquitoes examined belonged to An. sinensis, confirming that there was not any other species of Anopheline mosquitoes of Hyrcanus Complex in Yongcheng city [72, 73].
The abundance of female culicine species and An. sinensis per baited trap at each study site
1The abundance of female culicine species and An. sinensis in the study sites of Yongcheng city, Henan Province, China
2The study sites
The species of mosquitoes
95% Confidence Interval for Mean
Cx. pipiens pallens
Cx. pipiens pallens
Cx. pipiens pallens
1The abundance of female culicine species and An. sinensis in bednet traps baited with different animal and human hosts in Yongcheng city, Henan Province, China
The species of mosquitoes
95% Confidence Interval for Mean
Cx. pipiens pallens
Cx. pipiens pallens
Cx. pipiens pallens
Cx. pipiens pallens
Cx. pipiens pallens
Cx. pipiens pallens
Host-seeking activity of female culicine species and An. sinensis
As to the host-seeking activity of female Cx. tritaeniorhynchus, the results of Univariate analysis of variance demonstrated that there were significant differences among the number of female Cx. tritaeniorhynchus captured at each bait [F (5,1105) = 8.117, P < 0.01], and at each bednet location [F(5,1105) = 3.413, P = 0.005], but no differences at each night [F(5,1105) = 1.648, P = 0.145]. There were no interactions between nights and locations of the bednet traps [F (3, 1105) = 0.031, P = 0.993], locations of the bednet traps and baits [F (5, 1105) = 1.782, P = 0.114], and there were interactions between nights and baits [F (3, 1105) = 2.233, P = 0.08]. As far as the host-seeking activity of female Cx. pipiens pallens is concerned, there were significant differences among the number of female Cx. pipiens pallens captured at each bait [F(5,1105) = 6.989, P < 0.01], at each night [F(5,1105) = 7.118, P < 0.01], and at each bednet location [F(5,1105) = 3.257, P = 0.006]. There were interactions between nights and locations of the bednet traps [F (3, 1105) = 4.170, P = 0.006], nights and baits [F (3, 1105) = 3.903, P = 0.009], and locations of the bednet traps and baits [F (5, 1105) = 3.015, P = 0.01]. There were significant differences among the number of female An. sinensis captured at each bait [F (5, 1105) = 13.161, P < 0.01], at each night [F (5, 1105) = 4.460, P < 0.01], but no differences of each bednet location [F (5, 1105) = 0.259, P = 0.90]. There were no interactions between nights and locations of the bednet traps [F (3, 1105) = 0.912, P = 0.434], nights and baits [F (3, 1105) = 1.961, P = 0.118], and locations of the bednet traps and baits [F (5, 1105) = 1.094, P = 0.362].
Multiple blood meals of female engorged An. sinensis highlighted by blood meal analysis
Origin of blood meal of engorged female An. sinensis in the study sites during the work period
1Origin of blood meal of engorged female
The research sites
Human bednet trap
Human bednet trap
Human bednet trap
Hourly host-seeking activity of female An. sinensis
Variations in the hourly catches of host-seeking female An. sinensis in bednet traps baited with different animal and human hosts during 17 nights in Yongcheng city, Henan Province, China
Interval for Mean
Variation in the density of female An. sinensis with environment variables
The distribution of the An. sinensis density, the average temperature, and the average relative humidity in the study sites
1The study sites
One-Sample Kolmogorov-Simirnov Test
2The An. sinensis density(mosquito/net/night)
The average temperature(°C)
The average relative humidity (%)
The An. sinensis density(mosquito/net/night)
The average temperature(°C)
The average relative humidity (%)
The An. sinensis density(mosquito/net/night)
The average temperature(°C)
The average relative humidity (%)
The results obtained from the current research demonstrated that the species composition of mosquitoes in Yongcheng city includes Cx. tritaeniorhynchus, Cx. pipiens pallens, An. sinensis, Ae. albopictus, and Ar. subalbatus(Coquillett). This was the first time that the species composition of mosquitoes was reported in Yongcheng city. Though Cx. tritaeniorhynchus, Cx. pipiens pallens, Ae. albopictus, and Ar. subalbatus(Coquillett) are not vectors of P. vivax malaria, these species could be considered as the major nuisance on the basis of their high proportions. The influence of culicine species, especially Cx. tritaeniorhynchus and Cx. pipiens pallens should not be ignored, since Cx. tritaeniorhynchus is the primary vector of JE virus, and Cx. pipiens pallens is the primary vector of JE virus and filariasis in China . The ribosomal DNA PCR assay revealed that An. sinensis was the sole species of Hyrcanus Complex in identified female Anopheline mosquitoes in the study sites. The results of this study were consistent with those reported earlier in China [5, 25, 27]. Based on previous researches and results of the current PCR assay, it was probable that there weren't any other species of Anopheline mosquitoes of Hyrcanus Complex except for An. sinensis present in Yongcheng city.
Mosquito host-seeking behavior can be studied by different methods [30, 33, 74–77]. In the northeastern USA, host-seeking activities and avian host preferences of mosquitoes associated with West Nile virus (WNV) transmission were studied by a custom-designed trap baited with dry ice . To study potential WNV vectors, horse- and bird-baited traps and HLC methods were carried out weekly from May to October 2004 at two Camargue sites . In the Toledo District, Belize, Central America, host-feeding preferences of Anopheline species were collected by manual aspiration, mechanical aspiration, and a vehicle-mounted trap . In the present study, baited bednet traps [34, 79] were adopted to study the host-seeking behavior of culicine species and An. sinensis. The current study was similar to a study in Bernalillo County, New Mexico. Hosts used in their study were calves, chickens, dogs, and horses . The rationale for using baited bednet traps in the current study was that they would separate the zoophilic and the anthropophilic species while other methods of collection, such as HLC and IRS may not be suitable to use for this purpose.
Latin square design (LSD) was employed in the present study because the experimental set-up consisted of several baits tested on 5-6 experimental nights. Previous studies had established that the LSD would greatly reduce potential bias related to the location and alternated position of the baited-bednet samplers, allowing comparison of the attractiveness of different hosts to the mosquitoes in the field [34, 46].
The results of variance analysis demonstrated that there were significant differences among the abundance of Cx. tritaeniorhynchus for each bait, the abundance of An. sinensis for each bait, but no differences of female Cx. pipiens pallens for each bait. There were some major characteristics, which might influence the abundance of female Cx. tritaeniorhynchus and An. sinensis in these villages. First, during the study period, the locations of the bednet traps of Renhu village were near to a wide canal and many ponds (harboring endogenous lotus). High density of culicine and Anopheline mosquito larvae were observed from these breeding sites. In another experiment concerning breeding sites of Anopheline mosquitoes in 2010, Renhu village had more breeding sites of Anopheline mosquitoes than the other two villages. Second, the number of animal baits from high to low was consistent with the abundance of collected female culicine species and An. sinensis in the study sites. Differences in the availability of blood meals for female culicine species and female An. sinensis might exist within the study villages. Third, the locations selected for our study were sampled at different periods. This could possible explain the observed differences in the number of both Culicine and Anopheline mosquitoes caught between the villages. Fourth, the local selective pressures within which mosquitoes thrive might be added to the variability of the results.
By far, field studies have revealed that each mosquito species had its own host preference [81, 82]. Pair wise comparison showed that the rank of abundance of host-seeking female Cx. tritaeniorhynchus and An. sinensis for each bait from high to low was pigs, goats, calves, dogs, humans, and chickens, respectively. There was a consistent trend of the rank of abundance of host-seeking activity between female Cx. tritaeniorhynchus and An. sinensis. In Thailand, An. sinensis was almost entirely zoophilic in comparative biting tests involving man and cow; almost none of them were attracted to man . This phenomenon could be explained by an opportunistic feeding behavior present in these species. The anti-vector behavior at the community level, namely practices often used by local people for protection from mosquitoes, also had some influence on the host-seeking behavior of these species. Olfaction may be the major sensory modality involved in the resource searching behavior of insects . In mosquitoes, it is mainly exploited in host-seeking and finding a suitable place for oviposition. Differences in host-preference in the current study were likely to be reflected in their response to different host odors offered [85–88]. However, the focus on the current research was only one aspect of behavior in mosquitoes and cannot fully explain the dramatic re-emergence of malaria in the Huang-Huai River region of central China. Zhou et al  suggested that the spatial distribution between malaria cases and water-body, the changing of meteorological factors, and the increasing vectorial capacity and basic reproductive rate of An. sinensis were the possible determinants of malaria re-emergence in these areas.
In the current study, successful amplification was obtained by template DNA from the whole blood and female blood-fed An. sinensis in the research villages. All bednet traps sampled approximately the same proportions of female blood-fed An. sinensis. The results of multiplex PCR assay showed that only 3 blood meals were human in origin, while most of blood meals originated from domestic animals, such as pigs, calves, and goats. The overall HBI calculated from this study including mixed blood meals was 2.94% and 3.70% when compared with only the single blood meal. Similar studies were reported in Japan in 1951 ; in north Kyonggi-do in 1962 (1.7%), in 1999 (0.7%), and in 2000 (0.8%) . Though the HBI of these tests was very low, they readily fed on humans in high numbers where domestic animals were not nearby for feeding . The HBI of the current study was higher than that of these findings mentioned above. Gonotrophic discordance, or taking multiple blood meals during the gonotrophic cycle, has been reported in many different mosquito taxa. In these studies, approximately 10% to 40% of blood-fed field specimens contained multiple blood meals [92–99]. Amplification of template derived from mosquito abdomens containing mixed blood meals confirmed that multiple blood meals from different mammals could be detected in a single mosquito. Our studies demonstrated that high proportions of mixed blood meals were encountered (21/102), and cryptic blood meals were likely to be more numerous. Two to three sources of blood meal were detected from the abdomen of female blood-fed An. sinensis, and this phenomenon could be explained by a modification of the traditional view of the gonotrophic cycle . This host-feeding behavior can influence pathogen transmission through increased frequency of vector-human contact, or possibly reduce vector-human contact if some blood meals are taken from alternative mammalian hosts . In the current study, we failed to identify the origin of the blood meal in 11 out 102 field specimens examined. Insufficiency in host DNA concentrations due to the low blood meal volume in some mosquito specimens is presumed to be the cause of this failure. In addition, the process of blood digestion may have denatured host DNA, or the mosquito may have fed on an animal not included in the diagnostic assay .
The current study demonstrated that the host-seeking activity of female An. sinensis showed two peaks. The primary host-seeking activity occurred between 19:00 and 21:00 and a smaller peak between 4:00 and 5:00. It reported that An. sinensis bites man and animals soon after dark and throughout the night, with a peak generally in the first quarter of the night . In east China, its peak was from 19:00 to 21:00 . Chow (1949) found that the peak of An. sinensis biting buffalo in Chongqing was from 20:00 to 22:00. Zhou et al  reported that the biting time of An. sinensis was from 19:30 to 24:00 locally, with a peak time from 21:00 to 24:00 in Yongcheng city. The primary peak time in the present study was earlier than that of Zhou' s report  and consistent with a previous study in east China . This pattern suggested that vector abatement directed at adult An. sinensis would be most effective if initiated from 19:00 to 21:00, namely before human host go to bed at night.
Previous studies have shown that meteorological factors, such as the average temperature, rainfall, and relative humidity have a certain correlation to mosquito density in the corresponding period [101–105]. Temperature was found to be the most important environmental factor, followed by rainfall and relative humidity in the Delphi evaluation. However, relative humidity was found to be more important than rainfall and temperature in the ranking list according to the three single environmental factor regression models . Paaijmans et al  believed that the incubation period for malaria parasites within the mosquito is exquisitely temperature-sensitive, and temperature is a major determinant of malaria risk. Yang et al  found that the distribution of the mosquito vector was mainly related to relative humidity, which determined the extent of malaria transmission. In the current study, there were no correlations between the density of female An. sinensis and the average temperature in the study sites, while significant correlations between the density of female An. sinensis and the average relative humidity (r = 0.859, P = 0.029) were observed in Wangshanzhuang village. It could be explained due to the average relative humidity affecting the distribution and breeding of An. sinensis.
Care needs to be taken in interpreting the results of this study. Several factors may have affected the variability in the abundance and host-seeking behaviour of the culicine mosquitoes and An. sinensis in our study. First, biases inherent in the trapping method may have affected the observed number and species composition of mosquitoes within baited traps. Some mosquitoes may fly out from the gaps under the baited bednet traps more easily than others, resulting in some species being underrepresented. Second, the physiological status and infected proportions of mosquitoes were not recorded in the field; therefore, the importance of gravid female An. sinensis is not emphasized in the current report. Third, the abundance of Ae. albopictus, Ar. subalbatus (Coquillett), and other mosquito species in each site were not calculated because they were poorly represented in our samples. Further research should be undertaken using this multiplex PCR protocol for the identification of the origin of blood meals of female blood-fed An. sinensis, in addition, further studies are needed to better characterize the malaria vector and its respective role in malaria transmission. An olfactometry assay could be conducted in the future to clarify if behavior of An. sinensis is not directional and possibly provide an indication of how this species will behave in different circumstances. The origin of blood meals of Cx. tritaeniorhynchus could also be studied in the future because of its high proportions and epidemiologic implications .
Given the observed data in the studied research sites, the epidemiologic implications of culicine species, especially Cx. tritaeniorhynchus, could not be ignored on the basis of their high proportions. An. sinensis was the sole potential vector of P. vivax malaria in Yongcheng city. Pigs, goats, and calves had a greater tendency to attract both female Cx. tritaeniorhynchus and female An. sinensis than dogs, humans, and chickens at night. High proportions of mixed meals of female engorged An. sinensis were encountered, and cryptic meals were likely to be more numerous. When it comes to the host-seeking activity of female An. sinensis, 19:00~21:00 was the primary peak while 4:00~5:00 was the relatively smaller peak at night. These aspects mentioned above are of great importance to the understanding of vector biology of culicine species and An. sinensis, and the design of effective vector control strategies. These findings should be considered in the implementation of malaria elimination campaigns in China, especially in malaria re-emergence areas.
List of abbreviations used
polymerase chain reaction
human landing catch
indoor residual spray
human blood index.
This work is financially supported by the Major National Science and Technology Projects of China awarded #2008ZX10004-010 and the National Basic Research Program of China (973 Program) awarded #2012CB955500. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript, and the content is solely the responsibility of the authors and does not necessarily represent the official views of the Major National Science and Technology Projects of China and the National Basic Research Program of China (973 Program). We sincerely thank the anonymous referees and Prof. Chris Arme for their series of excellent comments and suggestions. We sincerely thank Yu-Jing Liu, Ph.D. from Northwest A&F University for her enthusiastic offering to help embellish the language of the revised version. We also sincerely thank Robert J. Novak, Ph.D. from University of South Florida who gave us guidance in the field in Yongcheng city. The authors would like to thank Wei-Zhong Yang from China CDC who helped in the guidance of the experiment. The authors would like to thank Feng-Xia Meng, Liang Lu, Hai-Xia Wu, Gui-Chang Li, Dong-Mei Li, Jun Wang and Xiu-Ping Song at Department of Vector Biology and Control, ICDC, China CDC who helped in conducting of the experiments. The authors would like to thank Zhen-Rong Cui, Yang Shen, Yun-Jun Su, Ming-Jie Zhu, Hai-Shan Wang and Guang-Xu Zhou from Yongcheng CDC who participated in mosquito collections, Ke-Hua Peng, Qing-Lin Zhu, and Heng-Chao Xie from township hospital who helped in organizing the experiments and capturing mosquitoes at night. And lastly, we are again grateful to the village doctors of the research sites who participated completely in this project.
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