Table 2 Primers used for PCR amplification and sequencing
From: Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes?
Primer name | Cytochrome oxidase subunit 1 – primer sequence | Forward or Reverse | Source |
|---|---|---|---|
Asmit1 (AT1) | 5' TTT TTT GGG CAT CCT GAG GTT TAT 3' | Forward | [26] |
Asmit2 (AT2) | 5' TAA AGA AAG AAC ATA ATG AAA ATG 3' | Reverse | [26] |
CO1 (LC1490) | 5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' | Forward | [18] |
CO2 (HCO2198) | 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' | Reverse | [18] |
BulCox1 (BC1) | 5' TTT TTG GWG TTT GAT GTG G 3' | Forward | NHM – current project |
BulCox2 (BC2) | 5' TGT GGT CTG GTA GGW ACC GG 3' | Forward | NHM – current project |
BulCox3 (BC3) | 5' CGT GGA AAW CTT ATA TCW GGW GC 3' | Reverse | NHM – current project |
BulCox4 (BC4) | 5' GCW CCW GAT ATA AGW TTT CCA CG 3' | Forward | NHM – current project |
BulCox5 (BC5) | 5' CCT TTA AGA GGN CCT ATT GC 3' | Forward | NHM – current project |
BulCox6 (BC6) | 5' CAA TAA ACC CTA AAA TYC C 3' | Reverse | NHM – current project |
BulCox7 (BC7) | 5' GCA ATA GGT CCT CTT AAA GG 3' | Reverse | NHM – current project |
BulCox8 (BC8) | 5' GTA ATA AAA TTA ATW GCA CCT AAA A 3' | Reverse | NHM – current project |
BulCox9 (BC9) | 5' CCW CCT TCA TTT ATT TT 3' | Forward | NHM – current project |
BulCox10 (BC10) | 5' GCT AAA TGT AAA G 3' | Reverse | NHM – current project |
BulCox11 (BC11) | 5' TTT TGG DRT YTG RTG YGG 3' | Forward | NHM – current project |
BulCox12 (BC12) | 5' GCG TTG ACT CTT TTC AAC 3' | Forward | NHM – current project |
BulCox13 (BC13) | 5' CWT TRT AYW TAA TTT TTG G 3' | Forward | NHM – current project |
BulCox14 (BC14) | 5' GGA AAT CAG TAM AYA AAA CCA GC 3' | Reverse | NHM – current project |
BulAsmit3 (BAT3) | 5' CAT AAT GAA AAT GAG CAA CTA C 3' | Reverse | NHM – current project |
BulAsmit4 (BAT4) | 5' CAT AAT GAA AAT GAG C 3' | Reverse | NHM – current project |
Primer name | Second internal transcribed spacer of the ribosomal gene complex – primer sequence | Forward or Reverse | Source |
ETTS1 | 5' TGC TTA AGT TCA GCG GGT 3' | Reverse | [27] |
ETTS10 | 5' GCA TAC TGC TTT GAA CAT CG 3' | Forward | [27] |