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Table 2 Primers used for PCR amplification and sequencing

From: Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes?

Primer name Cytochrome oxidase subunit 1 – primer sequence Forward or Reverse Source
Asmit1 (AT1) 5' TTT TTT GGG CAT CCT GAG GTT TAT 3' Forward [26]
Asmit2 (AT2) 5' TAA AGA AAG AAC ATA ATG AAA ATG 3' Reverse [26]
CO1 (LC1490) 5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward [18]
CO2 (HCO2198) 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' Reverse [18]
BulCox1 (BC1) 5' TTT TTG GWG TTT GAT GTG G 3' Forward NHM – current project
BulCox2 (BC2) 5' TGT GGT CTG GTA GGW ACC GG 3' Forward NHM – current project
BulCox3 (BC3) 5' CGT GGA AAW CTT ATA TCW GGW GC 3' Reverse NHM – current project
BulCox4 (BC4) 5' GCW CCW GAT ATA AGW TTT CCA CG 3' Forward NHM – current project
BulCox5 (BC5) 5' CCT TTA AGA GGN CCT ATT GC 3' Forward NHM – current project
BulCox6 (BC6) 5' CAA TAA ACC CTA AAA TYC C 3' Reverse NHM – current project
BulCox7 (BC7) 5' GCA ATA GGT CCT CTT AAA GG 3' Reverse NHM – current project
BulCox8 (BC8) 5' GTA ATA AAA TTA ATW GCA CCT AAA A 3' Reverse NHM – current project
BulCox9 (BC9) 5' CCW CCT TCA TTT ATT TT 3' Forward NHM – current project
BulCox10 (BC10) 5' GCT AAA TGT AAA G 3' Reverse NHM – current project
BulCox11 (BC11) 5' TTT TGG DRT YTG RTG YGG 3' Forward NHM – current project
BulCox12 (BC12) 5' GCG TTG ACT CTT TTC AAC 3' Forward NHM – current project
BulCox13 (BC13) 5' CWT TRT AYW TAA TTT TTG G 3' Forward NHM – current project
BulCox14 (BC14) 5' GGA AAT CAG TAM AYA AAA CCA GC 3' Reverse NHM – current project
BulAsmit3 (BAT3) 5' CAT AAT GAA AAT GAG CAA CTA C 3' Reverse NHM – current project
BulAsmit4 (BAT4) 5' CAT AAT GAA AAT GAG C 3' Reverse NHM – current project
Primer name Second internal transcribed spacer of the ribosomal gene complex – primer sequence Forward or Reverse Source
ETTS1 5' TGC TTA AGT TCA GCG GGT 3' Reverse [27]
ETTS10 5' GCA TAC TGC TTT GAA CAT CG 3' Forward [27]