Minimum Spanning Networks (MSN). Haplotypes are coded according to the population group within which they are found. Black circles represent shared haplotypes and colored circles represent haplotypes unique to a population within a group. Size of circle is proportional to the haplotypic frequency, and each cross bar indicates one nucleotide substitution between haplotypes. Dashed lines indicate alternative branching between haplotypes. (A) MSN obtained from all 34 haplotypes from Lu. longipalpis s.l. used in this study. The black circles are haplotypes shared by all population groups. Colored code: group <10°S (green); and group >10°S (red). (B) MSN for group <10°S: Black circles are haplotypes shared by populations within this group. Colored circles are haplotypes from Juazeiro (green), Natal (light blue), Itamaracá (grey), João Pessoa (orange), Patos (white) and Calumbi (dark blue). (C) MSN for group >10°S: Black circles are haplotypes shared by populations within this group. Colored circles are haplotypes specific to Pancas (yellow), Monte Santo (grey) and Feira de Santana (pink).