Figure 1

The use of trypomastigotes give better specificity than epimastigotes by IFA. Indirect immunofluorescence assay using Trypanosoma cruzi and Trypanosoma rangeli trypomastigote or epimastigote forms as antigens and serum (1:80) from a chronic chagasic patient with the cardiac form of the disease. Negative controls consist of epimastigotes and non-infected patient serum (1:40).