Western blots. A. Flagellar proteins (5.108 flagella, Fg) probed with Mab 22. B. N-terminal Histidine-tagged proteins expressed in E. coli (2.107 bacteria/well) and probed with anti-polyhistidine (upper panel) and Mab 22 (lower panel). Non-induced bacteria (-), 3 hr IPTG induction (+). C. Specificity of the affinity purified polyclonal anti-p99. In upper and lower panels PI: Pre-immune serum 1:1,000. I: Immune serum 1:500, AP: affinity purified immune serum 1:1000. WC: T. brucei whole cells (107/well). D. p99 RNAi whole cells (4.107/well) probed with the affinity purified immune serum anti-p99 1:500 and anti-PFR2 L8C4 monoclonal antibody (1:1,000) as loading control. Non-induced cells (-), Induced cells (+), Wild-type cells (WT).