Figure 2

An alignment of the inferred amino acid sequences of RIO1 proteins from Trichostrongylus vitrinus and other nine selected species. These nine species include Caenorhabditis elegans (accession no. AAC17564), C. briggsae (CAE60574), Brugia malayi (EDP30009), Homo sapiens (NP_113668), Mus musculus (NP_077204), Danio rerio (NP_998160), Drosophila melanogaster (NP_648489), Arabidopsis thaliana (NP_180071) and Schizosaccharomyces pombe (CAA15723). Amino acids predicted to be involved in the ATP binding motif and active sites are boxed. Identical amino acids are marked with asterisks. Predicted subdomains I-XI (see Fig. 1B; [7]) are marked above the alignment. Alpha-helices A-I (shaded) or beta-sheet structures (underlined) [1] are indicated. Flexible loop, hinge and metal-binding loop were identified (see Fig. 1D in [40]). Dashes indicate gaps in the sequence, included for alignment purposes.