Yellow fluorescent trypanosomes recovered from saliva or glands. A. Yellow epimastigote from fly salivary gland dissected at 13 days stained with Hoechst 33342. B. Trypanosomes in a salivary exudate sample obtained from an infected fly at day 14; main picture shows the unfixed trypanosomes by fluorescence microscopy and insets show Hoechst 33342 and brightfield images. B1 are unattached epimastigotes and the yellow epimastigote has two kinetoplasts; B2 appears to be a multinucleate cell. Scale bar = 10 μm.