The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei

Parasites & Vectors

Table 2 Genotypes and phenotypes of hybrid clones and parents

Genotype (no. of clones)	Fluorescence	Microsatellite alleles			Maxicircle type (no. of clones)	DNA content
		II-PLC	XI-53	III-2
J10 mRFP	Red	ab	aa	ab	J10	2N
1738 GFP	Green	cd	bc	cd	1738	2N
SG1
I (3)	Green	bd	ac	ac	J10	2N
II (1)	Green	bd	ac	bc	1738	2N
III (1)	Green	bc	ac	bc	1738	2N
IV (5)	None	bd	ac	ac	1738	2N
V (1)	None	bd	ac	ac	mix	2N
VI (1)	None	bd	ab	ac	1738	2N
VII (1)	None	bd	ac	ad	1738	4N + 2N
VIII (1)	None	ac	ab	ad	J10	2N
IX (3)	None	bd	ac	ac	1738	2N
X (2)	None	bd	ab	bc	1738	2N
XI (1)	Red	bc	ac	ac	1738	2N
XII (1)	Red	ad	ac	bd	1738	3N
XIII (3)	Red	ad	ac	bc	1738	4N + 2N
XIV (1)	Yellow	bd	ab	ad	J10	4N + 2N
XV (8)	Yellow	ad	ac	bd	1738	4N + 2N
SG3
XVI (4)	None	bc	ac	ac	1738 (3), mix (1)	2N
XVII (1)	None	ad	ab	bc	1738	4N + 2N
XVIII (1)	Red	bc	ab	bc	J10	2N
XIX (1)	Red	ab	aa	ad	1738	2N
XX (1)	Yellow	bc	ab	bd	mix	2N
SG18
XXI (3)	None	bc	ab	bc	J10 (2), 1738 (1)	2N
XXII (1)	None	ac	ab	bd	1738	4N
XXIII (1)	None	bc	ab	ac	J10	2N
XXIV (1)	Red	ac	ac	bc	1738	4N
SG22
XXV (9)	Green	bc	ac	ad	J10 (6), mix (3)	2N
XXVI (2)	None	ac	ab	ad	J10	2N
XXVII (2)	Red	bd	ac	bd	J10	4N + 2N
XXVIII (1)	Red	ac	ab	bc	J10	4N + 2N
XXIX (2)	Red	bc	ac	bc	mix	2N

ISSN: 1756-3305