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Table 1 Primer names, amplification conditions and the expected product sizes for each of the Species-specific primers.

From: Comparative evaluation of three PCR base diagnostic assays for the detection of pathogenic trypanosomes in cattle blood

Primer name Primer sequence Amplification conditions Product size
TCS(F)ILO344 5'-CGA GAA CGG CAC TTT GCG A-3' 94°C for 3 min, 94°C for 30 sec, 60°C for 30 sec, 72°C for 30 sec, 72°C for 5 min (30 cycles) 316 base pairs
TCS(R)IL0345 5'-GGA CAA ACA AAT CCC GCA CA-3'   
TCK(F)ILO963 5'-GCG GCA GGT CGA CGG ATC-3' 94°C for 7 min, 94°C for 1 min, 55°C for 1 min, 72°C for 2 min, 72°C for 5 min (30 cycles) 294 base pairs
TCK(R)IL0968 5'-CCC TCG AGA ACG AGC A-3'   
TVW-1 5'-CTG AGT GCT CCA TGT GCC AC-3' 94°C for 3 min, 94°C for 30 sec, 60°C for 30 sec, 72°C for 30 sec, 72°C for 5 min (30 cycles) 150 base pairs
TVW-2 5'-CCA CCA GAA CAC CAA CCT GA-3'   
TBR-1 5'-CGA ATG AAT AAA CAA TGC GCA GT-3' 94°C for 3 min, 94°C for 1 min, 55°C for 1 min, 72°C for 1 min, 72°C for 5 min (30 cycles) 177 base pairs
TBR-2 5'-AGA ACC ATT TAT TAG CTT TGT GC-3'   
  1. TCS – Trypanosoma congolense savannah subtype
  2. TCK – Trypanosoma congolense kilifi subtype
  3. TVW – Trypanosoma vivax
  4. TBR – Trypanosoma brucei