Table 1 Primer names, amplification conditions and the expected product sizes for each of the Species-specific primers.

TCS(F)ILO344

5'-CGA GAA CGG CAC TTT GCG A-3'

94°C for 3 min, 94°C for 30 sec, 60°C for 30 sec, 72°C for 30 sec, 72°C for 5 min (30 cycles)

316 base pairs

TCS(R)IL0345

5'-GGA CAA ACA AAT CCC GCA CA-3'

TCK(F)ILO963

5'-GCG GCA GGT CGA CGG ATC-3'

94°C for 7 min, 94°C for 1 min, 55°C for 1 min, 72°C for 2 min, 72°C for 5 min (30 cycles)

294 base pairs

TCK(R)IL0968

5'-CCC TCG AGA ACG AGC A-3'

TVW-1

5'-CTG AGT GCT CCA TGT GCC AC-3'

94°C for 3 min, 94°C for 30 sec, 60°C for 30 sec, 72°C for 30 sec, 72°C for 5 min (30 cycles)

150 base pairs

TVW-2

5'-CCA CCA GAA CAC CAA CCT GA-3'

TBR-1

5'-CGA ATG AAT AAA CAA TGC GCA GT-3'

94°C for 3 min, 94°C for 1 min, 55°C for 1 min, 72°C for 1 min, 72°C for 5 min (30 cycles)

177 base pairs

TBR-2

5'-AGA ACC ATT TAT TAG CTT TGT GC-3'