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Figure 1 | Parasites & Vectors

Figure 1

From: Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases

Figure 1

The pH profile of hemoglobinolytic activity in the I. ricinus gut tissue extracts. Gut tissue was dissected from partially engorged tick females (the 5-th day of feeding), washed from the gut contents and extracted. Fluorescently labeled AMC-hemoglobin was digested in vitro with gut extract at various pH values. The relative rate of degradation of the substrate was determined using the measurement of fluorescence in a continuous hemoglobinolytic assay. The error bars indicate standard deviations of the mean of triplicates.

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