Figure 1From: Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidasesThe pH profile of hemoglobinolytic activity in the I. ricinus gut tissue extracts. Gut tissue was dissected from partially engorged tick females (the 5-th day of feeding), washed from the gut contents and extracted. Fluorescently labeled AMC-hemoglobin was digested in vitro with gut extract at various pH values. The relative rate of degradation of the substrate was determined using the measurement of fluorescence in a continuous hemoglobinolytic assay. The error bars indicate standard deviations of the mean of triplicates.Back to article page