Skip to main content

Table 1 Degenerate PCR primers used for identification of genes encoding gut-associated peptidases of I. ricinus

From: Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases

peptidase

cathepsin B

cathepsin L

cathepsin C

cathepsin D

forward primer

5'CKTGYGGDTCTTGTTGGG 3'

5'CAGTGCGGVTCCTGCTGGGC3'

5'GTCGACACKCCYGCBAACTGCAC3'

5'TCACCCCAGCCKNCGTTCCA3'

reverse primer

5'GGCTCCTCTAAYYTBTGGGT 3'

5'GGCGATTCCGCACTGGTTGT3'

5'CCRTAGWAACCWCCMACATA3'

5'GAACACGTCNCCBARDATCCA3'

Sm* GenBank

CAC85211

CAA83538

CAA83543

AAB63442

Sm* forward domain

117SSCGSCWA124

123LCGSCWA129

20ADTPANC26

85GSSNLWV91

Sm* reverse domain

313WNSDWGD319

304NMCGIA309

337YIGGYY342

355WILGDIF361

annealing temperature

55°C

53°C

62°C

53°C

product length

608 bp

554 bp

998 bp

855 bp

sequenced clones

12

8

8

8

isoforms

2

1

1

1

  1. *- orthologues in Schistosoma mansoni