Figure 7
From: Malaria ookinetes exhibit multiple markers for apoptosis-like programmed cell death in vitro
A temporal profile of apoptosis markers displayed by ookinetes cultured in phosphate buffered saline. P. berghei ookinetes were collected after incubating gametocytaemic blood for 17 h and resuspended in PBS at 18 h. The proportion of apoptotic or dead cells out of 100 ookinetes was detected using acridine orange (AO: nuclear chromatin condensation), CaspaTag (CT: caspase activity) tests, at 5 different time points. Ookinetes with compromised membranes, and no detectable apoptosis markers were detected using propidium iodide(PI). Data represents a mean of 2 independent experiments, n = 100 per sample. Error bars: ± SEM. Different letters show significant differences in apoptosis from 18 h.