Table 1 List of primers used to validate oligoGEArray data.
From: Differential expression of Aedes aegypti salivary transcriptome upon blood feeding
Accession number | Primers (5' to 3') | PCR Amplification efficiencies | |
|---|---|---|---|
Angiopoietin-like protein | gi|94468605 | GGCAATACAAAGCGTTCTGC | 98.8% |
AAACCAAGCCAATACTCACCG | |||
Serine protease | gi|18568305 | TTGCTGTTGCTTCTTGCGT | 97.2% |
CACATTCGTTGCTCTGGCTA | |||
30 kDa protein | gi|61742032 | GCTTTCCGTTGGCAGACTAA | 98.8% |
AATCCGAGAAGTGTGGTCAGAC | |||
Mucin-like peritrophin | gi|94468595 | CAACAAGGAAACCACCTGTG | 98.03% |
CAATCCAATCCCATTTGCTC | |||
Glycine rich protein | gi|94469015 | CAAGTGCTATTGCCATGGTT | 97.6% |
GTGAGCAGCTGATCCAGACA | |||
Ribosomal protein (RpL5) | gi|94468377 | ATTACATTGCCGTCAAGGAG | 99.2% |
TCATCATCAGCGAGTTGGTC |