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Table 2 Differential expression of salivary transcriptome upon blood feeding.

From: Differential expression of Aedes aegypti salivary transcriptome upon blood feeding

  Up-regulated Down-regulated No change
  1 hpf 3 hpf 24 hpf 48 hpf 1 hpf 3 hpf 24 hpf 48 hpf 1 hpf 3 hpf 24 hpf 48 hpf
PUTATIVE SECRETORY PROTEINS
Secreted carrier-like proteins 0 1 2 0 2 2 2 5 10 9 8 7
Protease inhibitors 1 0 0 0 0 1 2 2 4 5 6 6
Serine proteases 2 2 1 0 1 1 1 3 6 6 7 6
Nucleotidases and others 0 0 0 0 0 0 0 1 4 10 10 9
Immunity related 4 4 4 2 0 0 2 3 16 16 14 15
Mucins and peritrophins 2 1 0 0 0 2 5 5 11 10 8 8
Unknown functions 6 4 2 3 0 4 6 9 45 46 45 42
HOUSE KEEPING FUNCTION
Housekeeping genes 6 42 15 8 37 28 56 65 306 270 266 264
Total 21 54 25 13 40 37 74 93 402 372 366 357
Total (%) 4.5 11.6 9.5 2.8 8.5 7.9 15.9 20.1 87 80.3 79 77.1
  1. Temporal expression of the salivary transcripts of Ae. aegypti after blood feeding was investigated using a customized oligoGEArray, and analysed using GEArray Expression Analysis suite 2.0 (SABiosciences). Normalization of data was performed using ribosomal protein (RpL5). Comparison of the two arrays (naive vs. post-blood fed) was performed using a fold (ratiometric analysis (x/y)). Three technical replicates were performed for each time point, and means were used for differential expression analysis. Genes were considered to be expressed differentially if they were found to be ≥ 2.0 fold (up-regulated) or ≤ 0.5 fold (down-regulated). Genes with less than two-fold difference and greater than half fold difference are grouped as no change.