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Table 2 Differential expression of salivary transcriptome upon blood feeding.

From: Differential expression of Aedes aegypti salivary transcriptome upon blood feeding

 

Up-regulated

Down-regulated

No change

 

1 hpf

3

hpf

24 hpf

48 hpf

1 hpf

3 hpf

24 hpf

48 hpf

1 hpf

3 hpf

24 hpf

48 hpf

PUTATIVE SECRETORY PROTEINS

Secreted carrier-like proteins

0

1

2

0

2

2

2

5

10

9

8

7

Protease inhibitors

1

0

0

0

0

1

2

2

4

5

6

6

Serine proteases

2

2

1

0

1

1

1

3

6

6

7

6

Nucleotidases and others

0

0

0

0

0

0

0

1

4

10

10

9

Immunity related

4

4

4

2

0

0

2

3

16

16

14

15

Mucins and peritrophins

2

1

0

0

0

2

5

5

11

10

8

8

Unknown functions

6

4

2

3

0

4

6

9

45

46

45

42

HOUSE KEEPING FUNCTION

Housekeeping genes

6

42

15

8

37

28

56

65

306

270

266

264

Total

21

54

25

13

40

37

74

93

402

372

366

357

Total (%)

4.5

11.6

9.5

2.8

8.5

7.9

15.9

20.1

87

80.3

79

77.1

  1. Temporal expression of the salivary transcripts of Ae. aegypti after blood feeding was investigated using a customized oligoGEArray, and analysed using GEArray Expression Analysis suite 2.0 (SABiosciences). Normalization of data was performed using ribosomal protein (RpL5). Comparison of the two arrays (naive vs. post-blood fed) was performed using a fold (ratiometric analysis (x/y)). Three technical replicates were performed for each time point, and means were used for differential expression analysis. Genes were considered to be expressed differentially if they were found to be ≥ 2.0 fold (up-regulated) or ≤ 0.5 fold (down-regulated). Genes with less than two-fold difference and greater than half fold difference are grouped as no change.