Parasites & Vectors

Table 1 Exposure conditions of the preserved samples and resulting PCRs produced from their gDNA extracts

From: Isolation and preservation of schistosome eggs and larvae in RNAlater^® facilitates genetic profiling of individuals

Sample sets	Conditions		PCR result
Lab.	Temperature°C	Time	COX1	ITS
1	26-28	3 days	✓	✓
2	26-28	1 week	✓	✓
3	26-28	2 weeks	✓	✓
4	37	3 days	✓	✓
5	37	1 week	✓	✓
6	37	2 weeks	✓	✓
7	4	6 months	✓	✓
8	-20	6 months	✓	✓
Field	Conditions		COX1	ITS
9	Tropical field (28-32°C) setting for 2 weeks + transport by plane as hold luggage		✓	✓
10	As in 9 and then storage at 4°C for up to 6 months		✓	✓
11	As in 9 and then storage at -20°C for up to 6 months		✓	✓

Each sample set 1-8 included 2 of each larval stage (cercariae, miracidia) and eggs preserved in 5 μl or 10 μl of RNAlater^® solution. Sample sets 9-10 contain 2 miracidia collected in the field and stored in 5 μl RNAlater^®. ✓ corresponds to 100% of the samples producing a positive PCR and sequences of good quality.

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ISSN: 1756-3305

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