Figure 3
T.b.brucei cells directly transferred from a flask culture to a 384 well plate and ATP detected with 15 μL luciferase based reagent. (A) Cell concentrations of T.b.brucei and detection by the luciferase based assay of 55 μL per well of over a dose range to 3 × 106 cells/mL with cells transferred directly from dilutions made from flask grown cultures. 15 μL of luciferase reagent was added for detection. The mean CPM was estimated over 4 replicates from two separate experiments. (B) There was a linear relationship between cell number and trypanosomes to 1 × 106 cells/mL (r2 = 0.99).