Immunohistological detection of B. malayi larvae in the uterus of adult B. malayi or in experimentally infected mosquitoes using polyclonal antibody to recombinant antigen Bm14. A The antibody labeled intra-uterine, stretched mf in an adult female B. malayi worm. B Multiple unlabeled mf in the midgut of Cx. pipiens at 2 h PI: C Overview of the head and parts of the thorax at 14 DPI negative for B. malayi larvae. D Abdomen of Cx. pipiens at 3 DPI without any visible developing B. malayi larvae (labeled or unlabeled). E Magnified, unlabeled mf in the midgut of Cx. pipiens at 2 h PI: F Section of a labeled L1 in the thorax muscles of Ae. aegypti-LVP at 1 DPI: G Section of an unlabeled L1 in the thorax of Ae. aegypti-RKF at 1 DPI: H Two sections of labeled larvae in the thorax of Ae. aegypti-LVP at 3 DPI: I Section of an unlabeled larva in Ae. aegypti-RKF at 3 DPI. J Section of an unlabeled larva in Ae. aegypti-RKF at 7 DPI (compare Fig. 4D). Scale bars: A-D 25 μm; E-F 10 μm.