2-D analysis of the LCE of L2. Silver-stained representative 2-D protein map of the LCE of L2 comprising pH gradient from 3 to 11 with the MWs ranking from 10 to 250 KDa (A). The silver-stained gel was used to align detected protein spots on the Western blots performed with serum of mice immunised with the LCE of L2 (B) and with horse serum (C). The circles indicate the spots that were immunolabelled with mice serum (B; spots 1,2,6,8,14–19,25,26) and the arrows indicate the spots that were immunolabelled with horse serum (C; spots 1–13 and 16–26). A total of 26 spots were isolated for further MS identification. The protein identification by MS is presented in Table 3.