2-D analysis of the LCE of L3. Silver-stained representative 2-D protein map of the LCE of L3 comprising pH gradient from 3 to 11 with the MWs ranking from 10 to 250 KDa (A). The silver-stained gel was used to align detected protein spots on the Western blots performed with serum of mice immunised with the LCE of L3 (B) and with horse serum (C). 39 spots immunolabelled with both sera were isolated for further MS identification. The protein identification by MS is presented in Table 4.