ITS-1 PCR product (graph A) and restriction patterns of Leishmania ITS-1. PCR product digested with Hhal (graph B). Lanes: 1 = L. infantum (MHOM/FR/LEM-75); 2 = L. tropica (MHOM/SU/74/K27); 3 = L. donovani(MHOM/IN/80/DD8); 4 = L. aethiopica ref (MHOM/ET/72/L100); 5 = L. major (MHOM/SU/73/5-ASKH); Lanes 6 - 9 are isolates from hyraxes: 6 = H11/SK, 7 = H2/SK, 8 = H32/SK, 9 = H02/SK; 10 = Negative control; M = 100 bp molecular marker ladder. N.B. H02 and H2 originate from a single hyrax; H02 had been passaged in hamster infection, re-isolated and processed by PCR/RFLP analysis.