Multiple species/isolate alignment of nucleotide sequence for exon 4 encoding codons 132-175. Primers for a multi-species pyrosequencing assay are indicated by arrows in the forward and reverse direction (167fs and 167r) and for the pyrosequencing primer (167seq). The first round forward PCR primer (167f) is not shown. The codon for F167Y is indicated in yellow. Sequence degeneracy is indicated in blue by the relevant nucleotide code: R = A/G, Y = C/T, K = G/T, M = A/C, S = C/G, W = A/T, B = C/G/T, D = A/G/T, H = A/C/T, V = A/C/G, N = any. Consensus sequences were generated from the following species; Cyathostomum catinatum (Cya cat), Cyclicocyclus nassatus (Cyc nas), Cylicocylus insigne (Cyc ins), Cylicocylcus ashworthi (Cyc ash), Cylicodontophorus bicoronatus (Cyd bic), Cylicocylcus calicatus (Cyc cal), Cylicostephanus goldi (Cys gol), Cylicocyclus leptostomum (Cyc lep), Cylicostephanus longibursatus (Cys lon), Cylicostephanus minutus (Cyc min) and for four L3 populations of unknown species, DL3, ILPHL3, JCL3 and WKL3. The 167 consensus (167 con) sequence is shown at the bottom of the alignment.