Relative message expression of co-stimulatory molecules and cytokines over 24 hrs. RAW 264.7 cells were unstimulated or stimulated for 1 hr with 5 μg SGE followed by 1, 3, 6 or 24 hrs of 100 ng/mL LPS or no LPS. Total RNA was extracted and real-time PCR performed to measure CD80, CD86, IL-10 and TNFα message levels. N = 3-5 independent experiments. * P < 0.05 ** P < 0.01.