Figure 1

Markers of apoptosis in Leishmania infantum promastigotes grown in vitro. A. Elongated L. infantum promastigotes grown in vitro under control conditions. B. Rounded L. infantum promastigotes grown in vitro in the presence of edelfosine. C. Flow cytometric analysis showing a bi-parametric plot of the forward and side scatter properties of L. infantum promastigotes grown in vitro under control conditions. D. Flow cytometric analysis showing a bi-parametric plot of the forward and side scatter properties of L. infantum promastigotes grown in vitro in the presence of edelfosine. E. L. infantum promastigotes labelled with the potentiometric probe TMRM showing specific staining of the mitochondrion. F. Flow cytometric analysis showing a mono-parametric plot of the TMRM-derived fluorescence emitted by L. infantum promastigotes grown at 26°C, 38°C and in the presence of the mitochondrial uncoupler CCCP. G. Electron microscopic image of two L. infantum promastigotes grown at 38°C. n = nuclei.