Cytokines and antibodies responses in mice vaccinated with CTL-, T-, and B-PDDV cocktails. (A-B) Cytokine responses following vaccination with C-, T- and B-PDDV multicomponent formulations. Seven days after the last immunization with either multicomponent PDDV formulations, 18K-PDDV, or PBS, splenocyte production of IFN-γ (A) and IL-4 (B) from each vaccine group was assessed. Splenocytes from each mouse were cultured (106/well) in triplicate for 48 h in the presence or absence of SWA (50 μg/ml). Supernatants were collected after 48 h and IFN-γ and IL-4 production was assessed. The data are expressed as the mean ± SD (n = 6 per group) of 12 mice from two independent experiments performed in triplicate wells. (C-E) Antibody responses following multicomponent vaccinations. IgG (C), IgG1 (D), and IgG2a (E) responses to SWA in immunized mice were determined by ELISA. Data are expressed as the mean ± SD (n = 6 per group) of 12 mice from two independent experiments performed in triplicate wells. *P < 0.05 and ***P < 0.001, compared to the 18K-PDDV and PBS groups.