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Table 1 Design of peptides used in the construction of the PDDVs.

From: The nature and combination of subunits used in epitope-based Schistosoma japonicum vaccine formulations affect their efficacy

Code Amino acid sequence of 18 Lys and the 18 Lys fused epitopes synthesized for PDDV formulations Oligonucleotide sequences used for plasmid construction Source Epitope type
18K KKKKKKKKKKKKKKKKKK None None None
C1 KKKKKKKKKKKKKKKKKKNLMKENKNL 5'-tcgacatgaatcttatgaaagaaaataagaatttag-3' Sj 97
(555-563)
CTL
   5'-aattctaaattcttattttctttcataagattcatg-3'   
C2 KKKKKKKKKKKKKKKKKKVRAVANDLK 5'-tcgacatggtaagagcggtggcaaatgacttaaaag-3' Sj 22.6
(134-142)
CTL
   5'-aattcttttaagtcatttgccaccgctcttaccatg-3'   
C3 KKKKKKKKKKKKKKKKKKATRLNNEVL 5'-tcgacatggcaactagattgaataatgaagttttgg-3' Sj 97
(672-680)
CTL
   5'-aattccaaaacttcattattcaatctagttgccatg-3'   
T2 KKKKKKKKKKKKKKKKKKITELEDVAERERLKA 5'-tcgacatgatcactgaacttgaagatgttgcagagagagaacgattaaaagcgg-3' Sj 97
(313-327)
Th-cell
   5'-aattccgcttttaatcgttctctctctgcaacatcttcaagttcagtgatcatg-3'   
T3 KKKKKKKKKKKKKKKKKKAKQYNICCKFKELLD 5'-tcgacatggctaagcaatataacatatgttgtaaatttaaagaacttctcgatg-3' Sj22.6
(111-125)
Th-cell
   5'-aattcatcgagaagttctttaaatttacaacatatgttatattgcttagccatg-3'   
B1 KKKKKKKKKKKKKKKKKKEQRLRERDEELESLRKSTTRTI 5'-tcgacatggaacagagacttagagaaagagatgaagaattagaaagtctaagaaagagtacaactagaacaatattg-3' Sj 97 (489-510) B-cell
   5'-aattcaatattgttctagttgtcatctttcttagactttctaattcttcatctctttctctaagtctctgttccatg-3'   
B2 KKKKKKKKKKKKKKKKKKWEVRREKEELKKDKEGKVSTL 5'-tcgacatgtgggaagtccgtcgtgaaaaggaagaattaaagaaagacaaggaaggcaaagtatccacacttg-3' Sj 22.6 (77-97) B-cell
   5'-aattcaagtgtggatactttgccttccttgtctttctttaattcttccttttcacgacggacttcccacatg-3'   
B3 KKKKKKKKKKKKKKKKKKRQEEEMKKAAEELAKLKEEFEK 5'-tcgacatgcgtcaggaagaagaaatgaagaaagcagccgaagaattagctaaactaaaagaagaatttgaaaaag-3' Sj 62 (167-188) B-cell
   5'-aattctttttcaaattcttcttttagtttagctaattcttcggctgctttcttcatttcttcttcctgacgcatg-3'