Figure 1

Expression of the Bm86 gene in unfed larvae, engorged nymphs, unfed males, and gut and ovaries of R. microplus females. Six biological replicates of larvae (approximately 100 larvae per sample), engorged nymphs (10 nymphs per sample), unfed males (10 males per sample), and individual ovaries and gut of partially engorged females (at day 5 of feeding) were analyzed by reverse transcriptase quantitative real-time PCR. The transcription level of Bm86 was calculate as relative quantity using the delta Ct comparative method normalized by the total amount of RNA used to generate the cDNA. Different letters (a, b) above the bars indicate significant statistical differences (one-way ANOVA, F = 11.456, Tukey post hoc test, P < 0.0001).