Skip to main content
Figure 5 | Parasites & Vectors

Figure 5

From: Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis

Figure 5

Expression and immunogenicity of bovipain-2. A. Expression of bovipain-2 in in vitro cultured merozoites. Aliquots of a merozoite suspension of the BboS2P strain of B. bovis were electrophoresed, blotted and exposed to antibodies against (1) recombinant bovipain-2, or (2) normal serum. Antibody binding was detected by incubation with peroxidase-labeled anti-mouse IgG and chemiluminiscence. Bands of 34 and 26 kDa correspond to the expected sizes of bovipain-2 pro-protein and mature protein, respectively. B. Immunogenicity of bovipain-2 in B. bovis-experimentally infected cattle. Stripes with blotted, partially purified bovipain-2 were incubated with sera from different bovines experimentally infected (day 63 p.i.) with the BboR1A strain (stripes 1-3) or the BboS2P strain of B. bovis (4-6); non infected bovine sera (8-9); or a monoclonal antibody that reacts with the histidine tag of the recombinant protein (7). A band of 50-55 kDa was recognized in samples 1, 6 and 7 by reaction with peroxidase-labeled species-specific secondary antibodies, followed by chemiluminiscence detection.

Back to article page