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Figure 6 | Parasites & Vectors

Figure 6

From: Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis

Figure 6

Localization of bovipain-2 in merozoites. Erythrocytes infected with B. bovis strain BboS2P (A, D, E); B. bovis, strain RAD (B); B. bigemina, Mexico strain (C), or non infected erythrocytes (F) were incubated with murine anti-bovipain-2 antibodies (A, B, C, F); anti-B. bovis RAP-1 monoclonal antibody 23/70.174 (D) or pre-immune murine serum (E); followed by detection with FITC- (A,D,E,F) or Alexa Fluor 488- (B,C) labeled anti-murine IgG, and observation by epifluorescene, with 400 Ă— (A,D,E,F) or 1000 Ă— magnification (B,C). Bovipain-2-associated fluorescence was observed as a round spot inside merozoites and within the erythrocyte cytoplasm (A,B,C), different from the punctuated fluorescence associated to RAP-1 (D). No cross-reactivity with erythrocyte proteins was detected (F); and no unspecific reaction was observed with pre-immune mouse serum (E).

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