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Figure 4 | Parasites & Vectors

Figure 4

From: Dynamics of digestive proteolytic system during blood feeding of the hard tick Ixodes ricinus

Figure 4

Immunolocalization of cathepsin B (IrCB) in the gut of an Ixodes ricinus female during feeding. Panel A: Western blot analysis of authentic IrCB in gut homogenates electro-transferred onto a PVDF membrane. LMW - protein markers; UF - unfed ticks; 2 d - 2 days fed ticks (0.2 gut/lane); 4 d, 5 d, 6 d - 4, 5, 6 days fed ticks, respectively (0.1 gut/lane); FF - fully fed ticks (0.08 gut/lane). CBB - Coomassie stained membrane; Ab@IrCB - rabbit antibody against recombinant IrCB (Ra×IrCBIg, dilution 1:100). Secondary antibody - Swine anti-rabbit-peroxidase conjugate (1:1000). Panel B: Immunolocalization of IrCB in the gut of I. ricinus on semi-thin sections using affinity purified rabbit antibody Ra×IrCBACP (30 μg/ml). Secondary antibody - Goat anti-rabbit with conjugated Alexa Fluor® 488 (1:200). Merged images with DAPI nuclei staining; scale bars = 20 μm. Panel C: Control images without primary antibodies.

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