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Figure 2 | Parasites & Vectors

Figure 2

From: Mosquito cell line glycoproteins: an unsuitable model system for the Plasmodium ookinete-mosquito midgut interaction?

Figure 2

Lectin blotting with a range of lectins to An. stephensi 43 cell line glycoproteins with and without competitive sugar inhibitions. The position of molecular weight markers run concurrently are shown, values in kDa. Two μg of protein were loaded to each well. Figure 2A: Lane 1 SNA, and with 100 mM Sialic acid (lane 2); lane 3 RCAII, with 100 mM galactose (lane 4), with 100 mM melibiose (lane 5); lane 6 Con A, with 100 mM mannose (lane 7), with 100 mM glucose (lane 8); lane 9 PNA, with 100 mM galactose (lane 10), 100 mM melibiose (lane 11); lane 12 JAC, with 100 mm GalNAc (lane 13); lane 14 JAC, with 100 mM galactose (lane 15), 100 mM melibiose (lane 16). Figure 2B: Lane 1 AAL, with 100 fucose (lane 2); lane 3 WGA; with 100 mM GlcNAc (lane 4), lane 5 UEAI ; lane 6 MALII ; lane 7 PHA. Representative blot shown of 3-5 separate experiments.

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