Figure 5
Lectin blotting with a range of lectins to An. gambiae 55 cell line glycoproteins before and after in situ glycosidase treatments. The position of molecular weight markers run concurrently are shown, values in kDa. Two μg of protein were loaded to each well. Figure 5A: Lane 1 SNA, after neuraminidase (lane 2); lane 3 RCAII, after β-galactosidase (lane 4), after PNGase F (lane 5); lane 6 Con A, after PNGase F (lane 7), after Endo H (lane 8); after SBA (lane 9); after O-glycanase™ (lane 10), after PNGase F (lane 11); lane 12 DBA, after α AGA (lane 13), after PNGase F (lane 14); lane 15 JAC, after O-glycanase™ (lane 16), after PNGase F (lane 17). Figure 5B: Lane 1 AAL, after Endo H (lane 2), after PNGase A (lane 3); lane 4 Con A, after PNGase A (lane 5); lane 6 after periodate treatment. Representative blot shown of 3-5 separate experiments.