Figure 6
Standard curve showing MTT cleavage against number of ookinetes. Ookinete numbers were estimated on a haemocytometer. Ookinetes were then diluted in PBS and a two-fold serial manner, and MTT was(5 mg/ml) added to each dilution, and the mixtures vortexed and incubated for 4 h at 37°C. After incubation the resultant solutions were plated out on microwell plates. Figure 6A: Linear regression trend line is shown. Each point corresponds to the mean of six wells, bars indicate standard deviation of means. Trend line R2 = 0.9974. Figure 6B: The lower limit of the linear range of the standard curve was at an absorbance reading of 0.02 which corresponds to around 1500 ookinetes.