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Table 2 Summary of glycosidases and running conditions used for digestion of mosquito cell line glycoproteins.

From: Mosquito cell line glycoproteins: an unsuitable model system for the Plasmodium ookinete-mosquito midgut interaction?

Enzyme
(enzyme number)
Abbreviation Cleavage site Enzyme
Conc.
Incubation Buffer (pH) Reference
Peptide- N -glycosidase F
(EC 3.2.2.18)
PNGase F N-linked
oligosaccharides-Asn
50 U 0.5 M sodium phosphate (7.5) [59]
Peptide- N -glycosidase A
(EC 3.5.1.52)
PNGase A N-linked
oligosaccharides-Asn
2.5 mU 50 mM sodium citrate, sodium phosphate (5.0) [60]
Endo-β-glucosaminidase H
(EC 3.2.1.96)
Endo H Oligomannose/
hybrid oligosaccharides-Asn
50 U 0.5 M sodium citrate (5.5) [61]
Endo-α-acetylgalactosaminidase
(EC 3.2.1.92/3.2.1.110)
O-glycanase™ Galβ1-3GalNAcα1-Ser/Thr 82-112 mU 10 mM sodium citrate, sodium phosphate (6.0) [62]
α- N -acetylgalactosaminidase
(EC 3.2.1.49)
α-AGA GalNAcα1-3R/Ser/Thr 625 mU 100 mM sodium citrate, sodium phosphate (3.5-4) [63]
β-Galactosidase
(EC 3.2.1.23)
- Galβ1-3GlcNAc
-Glc/Man
4 mU 20 mM sodium citrate, sodium phosphate (6.0) [64]
Neuraminidase
(EC 3.2.1.18)
- Sialic acidα2-3,6,8R 25 mU 0.5 M sodium citrate (4.5) [65]