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Table 2 Summary of glycosidases and running conditions used for digestion of mosquito cell line glycoproteins.

From: Mosquito cell line glycoproteins: an unsuitable model system for the Plasmodium ookinete-mosquito midgut interaction?

Enzyme

(enzyme number)

Abbreviation

Cleavage site

Enzyme

Conc.

Incubation Buffer (pH)

Reference

Peptide- N -glycosidase F

(EC 3.2.2.18)

PNGase F

N-linked

oligosaccharides-Asn

50 U

0.5 M sodium phosphate (7.5)

[59]

Peptide- N -glycosidase A

(EC 3.5.1.52)

PNGase A

N-linked

oligosaccharides-Asn

2.5 mU

50 mM sodium citrate, sodium phosphate (5.0)

[60]

Endo-β-glucosaminidase H

(EC 3.2.1.96)

Endo H

Oligomannose/

hybrid oligosaccharides-Asn

50 U

0.5 M sodium citrate (5.5)

[61]

Endo-α-acetylgalactosaminidase

(EC 3.2.1.92/3.2.1.110)

O-glycanaseâ„¢

Galβ1-3GalNAcα1-Ser/Thr

82-112 mU

10 mM sodium citrate, sodium phosphate (6.0)

[62]

α- N -acetylgalactosaminidase

(EC 3.2.1.49)

α-AGA

GalNAcα1-3R/Ser/Thr

625 mU

100 mM sodium citrate, sodium phosphate (3.5-4)

[63]

β-Galactosidase

(EC 3.2.1.23)

-

Galβ1-3GlcNAc

-Glc/Man

4 mU

20 mM sodium citrate, sodium phosphate (6.0)

[64]

Neuraminidase

(EC 3.2.1.18)

-

Sialic acidα2-3,6,8R

25 mU

0.5 M sodium citrate (4.5)

[65]