Figure 2

Immunohistochemical reaction for antibodies bound to trophozoites in liver of hamsters infected with E. histolytica or E. dispar trophozoites. (a) Nodular lesion constituted by neutrophils and macrophages (*) and circumscribed by non-necrotic hepatic parenchyma (H). Vacuolizated E. dispar trophozoites with positive immunoreactivity for bound antibodies without contact to inflammatory cells (arrow). (b) Necrosis area (N) containing positive E. histolytica trophozoites (arrows). Immunoreactivity was also observed in injured hepatocytes (*). (c) Hepatic parenchyma containing positive reaction for antibodies on sinusoidal capillaries walls (arrow heads), injured hepatocytes (*) and E. histolytica trophozoites (arrows). (H) non-necrotic parenchyma. Bar = 20 μm.