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Figure 4 | Parasites & Vectors

Figure 4

From: Entamoeba histolytica and E. dispar trophozoites in the liver of hamsters: in vivo binding of antibodies and complement

Figure 4

Immunohistochemical reaction for the components C3 and C9 of the complement in liver of hamsters infected with E. histolytica or E. dispar trophozoites. (a) C9(+) trophozoites (arrows) present in the necrotic area (N) induced by E. histolytica infection. Note the presence of C9(+) trophozoites inside of the vessels (arrow heads). (H) non-necrotic parenchyma. Bar = 20 μm. (b) Hepatic necrosis (N) produced by E. dispar. C9(+) are observed in intact (arrows) and fragmented trophozoites (arrow heads). Bar = 20 μm. (c) Necrotic area produced by E. histolytica (N). Extensive areas of positive reaction for the C3 component of the complement are observed (*). (H) non-necrotic parenchyma. Bar = 100 μm. (d) Weak C3(+) hepatic parenchyma of hamsters inoculated with E. dispar (arrow heads). (N), necrotic zone. (H) non-necrotic parenchyma. Bar = 100 μm. (e) Amplification of the Figure 4d showing C3(+) hepatocytes (*) and fragmented trophozoites of E. dispar (arrow heads). Bar = 20 μm. (f) Positive control for C3 immunoreaction (*) using an ischemic/reperfused rat heart. Bar = 100 μm.

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